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We have previously shown that agents that activate the critical regulatory enzyme protein kinase C (PKC) slow cell migration during wound repair. Based on this observation and direct kinase measurements that demonstrate that dust extract from hog confinement barns (HDE) specifically activates the PKC isoforms PKC\u03b1 and PKC\u03b5, we hypothesized that HDE would slow wound closure time in airway epithelial cells. We utilized the human bronchial epithelial cell line BEAS-2B and transfected BEAS-2B cell lines that express dominant negative (DN) forms of PKC isoforms to demonstrate that HDE slows wound closure in BEAS-2B and PKC\u03b5 DN cell lines. However, in PKC\u03b1 DN cells, wound closure following HDE treatment is not significantly different than media-treated cells. These results suggest that the PKC\u03b1 isoform is an important regulator of cell migration in response to agricultural dust exposure.<\/jats:p>","DOI":"10.1152\/ajplung.00274.2007","type":"journal-article","created":{"date-parts":[[2007,10,13]],"date-time":"2007-10-13T00:53:53Z","timestamp":1192236833000},"page":"L1469-L1474","source":"Crossref","is-referenced-by-count":23,"title":["Hog barn dust slows airway epithelial cell migration in vitro through a PKC\u03b1-dependent mechanism"],"prefix":"10.1152","volume":"293","author":[{"given":"Rebecca E.","family":"Slager","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane S.","family":"Allen-Gipson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexi","family":"Sammut","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Art","family":"Heires","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"DeVasure","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanna","family":"Von Essen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debra J.","family":"Romberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd A.","family":"Wyatt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.079822"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00373.2005"},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Beckmann JD, Takizawa H, Romberger D, Illig M, Claassen L, Rickard K, Rennard SI.Serum-free culture of fractionated bovine bronchial epithelial cells. 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