{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:15:45Z","timestamp":1781540145665,"version":"3.54.5"},"reference-count":52,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:p> Alveolar barrier function depends critically on the claudin family tight junction proteins. Of the major claudins expressed by alveolar epithelial cells, claudin (Cldn)-3 and Cldn-4 are the most closely related by amino acid homology, yet they differ dramatically in the pattern of expression. Previously published reports have shown that Cldn-3 is predominantly expressed by type II alveolar epithelial cells; Cldn-4 is expressed throughout the alveolar epithelium and is specifically upregulated in response to acute lung injury. Using primary rat alveolar epithelial cells transduced with yellow fluorescent protein-tagged claudin constructs, we have identified roles for Cldn-3 and Cldn-4 in alveolar epithelial barrier function. Surprisingly, increasing expression of Cldn-3 decreased alveolar epithelial barrier function, as assessed by transepithelial resistance and dye flux measurements. Conversely, increasing Cldn-4 expression improved alveolar epithelial transepithelial resistance compared with control cells. Other alveolar epithelial tight junction proteins were largely unaffected by increased expression of Cldn-3 and Cldn-4. Taken together, these results demonstrate that, in the context of the alveolar epithelium, Cldn-3 and Cldn-4 have different effects on paracellular permeability, despite significant homology in their extracellular loop domains. <\/jats:p>","DOI":"10.1152\/ajplung.00299.2010","type":"journal-article","created":{"date-parts":[[2011,4,23]],"date-time":"2011-04-23T01:15:12Z","timestamp":1303521312000},"page":"L40-L49","source":"Crossref","is-referenced-by-count":100,"title":["Differential effects of claudin-3 and claudin-4 on alveolar epithelial barrier function"],"prefix":"10.1152","volume":"301","author":[{"given":"Leslie A.","family":"Mitchell","sequence":"first","affiliation":[{"name":"Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine,"},{"name":"Emory Alcohol and Lung Biology Center, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian E.","family":"Overgaard","sequence":"additional","affiliation":[{"name":"Division of 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