{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T18:08:36Z","timestamp":1789409316048,"version":"build-2803163510"},"reference-count":35,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p> IFN-\u03b3 production is upregulated in lung cells (LC) of bleomycin-treated C57BL\/6 mice. The present study characterizes the time course, cellular source, and regulation of IFN-\u03b3 expression in bleomycin-induced lung injury. IFN-\u03b3 mRNA in LC from bleomycin-treated mice peaked 3 days after intratracheal instillation. IFN-\u03b3 protein levels were increased at 6 days, as was the percentage of LC expressing IFN-\u03b3. CD4<jats:sup>+<\/jats:sup>, CD8<jats:sup>+<\/jats:sup>, and natural killer cells each contributed significantly to IFN-\u03b3 production. IL-12 mRNA levels were increased at 1 day in LC of bleomycin-treated mice. Anti-IL-12 and anti-IL-18 antibodies decreased IFN-\u03b3 production by these cells. To define the role of endogenous IFN-\u03b3 in the evolution of bleomycin lung injury, we compared the effect of bleomycin in mice with a targeted knockout mutation of the IFN-\u03b3 gene (IFN-\u03b3 knockout) and wild-type mice. At 14 days after intratracheal bleomycin, total bronchoalveolar lavage cell counts and lung hydroxyproline were decreased in IFN-\u03b3 knockouts compared with wild-type animals. There was no difference in morphometric parameters of fibrosis. Our data show that enhanced IFN-\u03b3 production in the lungs of bleomycin-treated mice is at least partly IL-12 and IL-18 dependent. Absence of IFN-\u03b3 in IFN-\u03b3 knockout mice does not increase pulmonary fibrosis. Endogenous IFN-\u03b3 may play a proinflammatory or profibrotic role in bleomycin-induced lung fibrosis. <\/jats:p>","DOI":"10.1152\/ajplung.00303.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:22:04Z","timestamp":1425414124000},"page":"L1255-L1262","source":"Crossref","is-referenced-by-count":57,"title":["Role of interferon-\u03b3 in the evolution of murine bleomycin lung fibrosis"],"prefix":"10.1152","volume":"285","author":[{"given":"Michael J.","family":"Segel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Izbicki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pazit Y.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reuven","family":"Or","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas G.","family":"Christensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shulamit B.","family":"Wallach-Dayan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael","family":"Breuer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"REF1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.12.6880"},{"key":"REF2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1996.tb32569.x"},{"key":"REF3","doi-asserted-by":"publisher","DOI":"10.1016\/0929-7855(95)00048-8"},{"key":"REF4","doi-asserted-by":"publisher","DOI":"10.1159\/000050488"},{"key":"REF5","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.1999.14c12.x"},{"key":"REF6","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.24.5.4064"},{"key":"REF7","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.22.5.f185"},{"key":"REF8","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.26.1.4285"},{"key":"REF9","doi-asserted-by":"publisher","DOI":"10.1378\/chest.97.6.1439"},{"key":"REF10","unstructured":"Elias JA, Jimenez SA, and Freundlich B. 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