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Transcriptional analysis and ELISA on bronchoalveolar lavage fluid revealed that the presence of the C allele correlated with more SP-B promoter activity and protein. There was approximately threefold difference in amounts of SP-B in bronchoalveolar lavage fluid from CA(\u221218) and AA(\u221218) individuals. By EMSA, Sp1 bound more tightly to the C allele sequence than to the A allele sequence, perhaps accounting for the differences in transcription. Genotyping of a normal volunteer population showed \u223c31% of the population were AA homozygotes, suggesting that these individuals produce less SP-B. Differences in amounts of SP-B resulting from the promoter SNP could affect the clinical presentation of pulmonary disease.<\/jats:p>","DOI":"10.1152\/ajplung.00307.2006","type":"journal-article","created":{"date-parts":[[2006,10,28]],"date-time":"2006-10-28T00:33:43Z","timestamp":1161995623000},"page":"L448-L453","source":"Crossref","is-referenced-by-count":15,"title":["The C\/A(\u221218) polymorphism in the surfactant protein B gene influences transcription and protein levels of surfactant protein B"],"prefix":"10.1152","volume":"292","author":[{"given":"Wendy K.","family":"Steagall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-Ping","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel","family":"Moss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01305.2005"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Bohinski RJ, Huffman JA, Whitsett JA, Lattier DL.Cis-active elements controlling lung-specific expression of human pulmonary surfactant protein B gene.J Biol Chem268: 11160\u201311166, 1993.","DOI":"10.1016\/S0021-9258(18)82105-6"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.16.1.8998078"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.17.7794"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1086\/315866"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(98)00060-X"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.99.13614779"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.01.00043401"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(98)00064-7"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1007\/BF02831993"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.152.5.7582290"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.1.20"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414134200"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.26.16104"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1006\/mgme.1998.2702"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0004.2000.580305.x"},{"key":"R17","unstructured":"Liu W, Bentley CM, Floros J.Study of human SP-A, SP-B and SP-D loci: allele frequencies, linkage disequilibrium and heterozygosity in different races and ethnic groups.BMC Genet4: 13\u201320, 2003."},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.278.3.L477"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.5.3083"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00011.2003"},{"key":"R21","doi-asserted-by":"crossref","unstructured":"Murata Y, Kim HG, Rogers KT, Udvadia AJ, Horowitz JM.Negative regulation of Sp1trans-activation is correlated with the binding of cellular proteins to the amino terminus of the Sp1trans-activation domain.J Biol Chem269: 20674\u201320681, 1994.","DOI":"10.1016\/S0021-9258(17)32046-X"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.161.3.9903153"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100570200"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1.1989.8.75"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000124872.55243.5A"},{"key":"R26","unstructured":"Schug J, Overton GC.TESS: Transcription Element Search Software. 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