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Although leucine-rich repeat-containing (LRRC) proteins have been identified as novel auxiliary \u03b3-subunits of the BK channel (BK\u03b3) in several cell types, its physiological roles in SMCs are unclear. The BK\u03b3 expression patterns in selected SM tissues were examined using real-time PCR analyses and Western blotting. The functional contribution of BK\u03b31 to BK channel activity was examined by whole cell patch-clamp in SMCs and heterologous expression systems. BK\u03b31 expression in mouse bronchial SMCs (mBSMCs) was higher than in other several SMC types. Coimmunoprecipitation and total internal reflection fluorescence imaging analyses revealed molecular interaction between BK\u03b1 and BK\u03b31 in mBSMCs. Under voltage-clamp, steady-state activation of BK channel currents at pCa 8.0 in mBSMCs occurred in a voltage range comparable to that of reconstituted BK\u03b1\/BK\u03b31 complex. However, this range was much more negative than in mouse aortic SMCs (mASMCs) or in HEK293 cells expressing BK\u03b1 alone and \u03b2-subunit (BK\u03b21). Mallotoxin, a selective activator of BK channel that lacks BK\u03b31, dose-dependently activated BK currents in mASMCs but not in mBSMCs. The abundant expression of BK\u03b31 in mBSMCs extensively facilitates BK channel activity to keep the resting membrane potential at negative values and prevents contraction under physiological conditions. <\/jats:p>","DOI":"10.1152\/ajplung.00331.2019","type":"journal-article","created":{"date-parts":[[2019,12,4]],"date-time":"2019-12-04T15:47:02Z","timestamp":1575474422000},"page":"L366-L375","source":"Crossref","is-referenced-by-count":11,"title":["Roles of LRRC26 as an auxiliary \u03b31-subunit of large-conductance Ca<sup>2+<\/sup>-activated K<sup>+<\/sup> channels in bronchial smooth muscle cells"],"prefix":"10.1152","volume":"318","author":[{"given":"Sayuri","family":"Noda","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 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