{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:01:27Z","timestamp":1790406087144,"version":"4.1.0"},"reference-count":32,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2003,5,1]]},"abstract":"<jats:p> Secretion of HCO[Formula: see text] by airway submucosal glands is essential for normal liquid and mucus secretion. Because the liquid bathing the airway surface (ASL) is acidic, it has been proposed that the surface epithelium may acidify HCO[Formula: see text]-rich glandular fluid. The aim of this study was to investigate the mechanisms by which intact distal bronchi, which contain both surface and glandular epithelium, modify pH of luminal fluid. Distal bronchi were isolated from pig lungs, cannulated in a bath containing HCO[Formula: see text]-buffered solution, and perfused continually with an aliquot of similar, lightly buffered solution (LBS) in which NaCl replaced NaHCO[Formula: see text] (pH 7 with NaOH). The pH of this circulating LBS initially acidified (by 0.053 \u00b1 0.0053 pH units) and transepithelial potential difference (PD) depolarized. The magnitude of acidification was increased when pH<jats:sub>LBS<\/jats:sub> was higher. This acidification was unaffected by luminal dimethylamiloride (DMA, 100 \u03bcM) but was inhibited by 100 nM bafilomycin A<jats:sub>1<\/jats:sub> (by 76 \u00b1 13%), suggesting involvement of vacuolar-H<jats:sup>+<\/jats:sup> ATPase. Addition of ACh (10 \u03bcM) evoked alkalinization of luminal LBS and hyperpolarization of transepithelial PD. The alkalinization was inhibited in HCO[Formula: see text]-free solutions containing acetazolamide (1 mM) and by DMA and was enhanced by bumetanide (100 \u03bcM), an inhibitor of Cl<jats:sup>\u2212<\/jats:sup> secretion. The hyperpolarization was unaffected by these maneuvers. The anion channel blocker 5-nitro-2-(3-phenylpropylamino)benzoate (300 \u03bcM) and combined treatment with DMA and bumetanide blocked both the alkalinization and hyperpolarization responses to ACh. These results are consistent with earlier studies showing that ACh evokes glandular secretion of HCO[Formula: see text] and Cl<jats:sup>\u2212<\/jats:sup>. Isolated distal airways thus secrete both acid and base equivalents. <\/jats:p>","DOI":"10.1152\/ajplung.00348.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T15:21:34Z","timestamp":1425396094000},"page":"L855-L862","source":"Crossref","is-referenced-by-count":29,"title":["Secretion of acid and base equivalents by intact  distal airways"],"prefix":"10.1152","volume":"284","author":[{"given":"S. K.","family":"Inglis","sequence":"first","affiliation":[{"name":"Tayside Institute of Child Health, University of Dundee, Dundee DD1 9SY, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Tayside Institute of Child Health, University of Dundee, Dundee DD1 9SY, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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