{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:05:46Z","timestamp":1791219946166,"version":"4.1.0"},"reference-count":44,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2002,4,1]]},"abstract":"<jats:p> We investigated the mechanism by which cAMP increases sodium transport in lung epithelial cells. Alveolar type II (ATII) cells have two types of amiloride-sensitive, cation channels: a nonselective cation channel (NSC) and a highly selective channel (HSC). Exposure of ATII cells to cAMP, \u03b2-adrenergic agonists, or other agents that increase adenylyl cyclase activity increased activity of both channel types, albeit by different mechanisms. NSC open probability ( P <jats:sub>o<\/jats:sub>) increased severalfold when exposed to terbutaline, isoproterenol, forskolin, or cAMP analogs without any change in NSC number. In contrast, terbutaline increased HSC number with no significant change in HSC P <jats:sub>o<\/jats:sub>. For both channels, the effect of terbutaline was blocked by propranolol and H-89, suggesting a protein kinase A (PKA) requirement for \u03b2-adrenergic-induced changes in channel activity. Terbutaline increased cAMP levels in ATII cells, but intracellular calcium also increased. Calcium sequestration with BAPTA blocked \u03b2-adrenergic-induced increases in NSC P <jats:sub>o<\/jats:sub> but did not alter HSC activity. These observations suggest that \u03b2-adrenergic stimulation increases intracellular cAMP and activates PKA. PKA increases HSC number and increases intracellular calcium. The increase in calcium increases NSC P <jats:sub>o<\/jats:sub>. Thus increased cAMP levels are likely to increase lung sodium transport regardless of which channel type is present. <\/jats:p>","DOI":"10.1152\/ajplung.00356.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:16:38Z","timestamp":1425413798000},"page":"L609-L620","source":"Crossref","is-referenced-by-count":76,"title":["\u03b2-Adrenergic regulation of amiloride-sensitive lung  sodium channels"],"prefix":"10.1152","volume":"282","author":[{"given":"Xi-Juan","family":"Chen","sequence":"first","affiliation":[{"name":"Departments of Pediatrics and Physiology and The Center for Cell and Molecular Signaling, Emory University School of Medicine, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas C.","family":"Eaton","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Physiology and The Center for Cell and Molecular Signaling, Emory University School of Medicine, Atlanta, Georgia 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