{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:50:01Z","timestamp":1772164201208,"version":"3.50.1"},"reference-count":42,"publisher":"American Physiological Society","issue":"1","funder":[{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute (NHBLI)","doi-asserted-by":"publisher","award":["HL94567"],"award-info":[{"award-number":["HL94567"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"HHS | NIH | National Cancer Institute (NCI)","doi-asserted-by":"publisher","award":["CA009535"],"award-info":[{"award-number":["CA009535"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2017,1,1]]},"abstract":"<jats:p>\n                    In many mammals, including humans, removal of one lung (pneumonectomy) results in the compensatory growth of the remaining lung. Compensatory growth involves not only an increase in lung size, but also an increase in the number of alveoli in the peripheral lung; however, the process of compensatory neoalveolarization remains poorly understood. Here, we show that the expression of \u03b1-smooth muscle actin (SMA)\u2014a cytoplasmic protein characteristic of myofibroblasts\u2014is induced in the pleura following pneumonectomy. SMA induction appears to be dependent on pleural deformation (stretch) as induction is prevented by plombage or phrenic nerve transection ( P &lt; 0.001). Within 3 days of pneumonectomy, the frequency of SMA\n                    <jats:sup>+<\/jats:sup>\n                    cells in subpleural alveolar ducts was significantly increased ( P &lt; 0.01). To determine the functional activity of these SMA\n                    <jats:sup>+<\/jats:sup>\n                    cells, we isolated regenerating alveolar ducts by laser microdissection and analyzed individual cells using microfluidic single-cell quantitative PCR. Single cells expressing the SMA ( Acta2) gene demonstrated significantly greater transcriptional activity than endothelial cells or other discrete cell populations in the alveolar duct ( P &lt; 0.05). The transcriptional activity of the Acta2\n                    <jats:sup>+<\/jats:sup>\n                    cells, including expression of TGF signaling as well as repair-related genes, suggests that these myofibroblast-like cells contribute to compensatory lung growth.\n                  <\/jats:p>","DOI":"10.1152\/ajplung.00383.2016","type":"journal-article","created":{"date-parts":[[2016,11,11]],"date-time":"2016-11-11T23:13:46Z","timestamp":1478906026000},"page":"L79-L88","source":"Crossref","is-referenced-by-count":12,"title":["Deformation-induced transitional myofibroblasts contribute to compensatory lung growth"],"prefix":"10.1152","volume":"312","author":[{"given":"Robert D.","family":"Bennett","sequence":"first","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology, Brigham &amp; Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra B.","family":"Ysasi","sequence":"additional","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology, Brigham &amp; Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willi L.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristian D.","family":"Valenzuela","sequence":"additional","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology, Brigham &amp; Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Tsuda","sequence":"additional","affiliation":[{"name":"Molecular and Integrative Physiological Sciences, Harvard School of Public Health, Boston, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saumyadipta","family":"Pyne","sequence":"additional","affiliation":[{"name":"Indian Institute of Public Health, Kavuri Hills, Madhapur, Hyderabad, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuqiang","family":"Li","sequence":"additional","affiliation":[{"name":"Fluidigm Corporation, South San Francisco, California; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonna","family":"Grimsby","sequence":"additional","affiliation":[{"name":"Broad Institute of Harvard and MIT, Cambridge, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prapti","family":"Pokharel","sequence":"additional","affiliation":[{"name":"Broad Institute of Harvard and MIT, Cambridge, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth J.","family":"Livak","sequence":"additional","affiliation":[{"name":"Fluidigm Corporation, South San Francisco, California; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maximilian","family":"Ackermann","sequence":"additional","affiliation":[{"name":"Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Blainey","sequence":"additional","affiliation":[{"name":"Broad Institute of Harvard and MIT, Cambridge, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven J.","family":"Mentzer","sequence":"additional","affiliation":[{"name":"Laboratory of Adaptive and Regenerative Biology, Brigham &amp; 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