{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:42:29Z","timestamp":1790383349530,"version":"4.1.0"},"reference-count":70,"publisher":"American Physiological Society","issue":"2","funder":[{"name":"NIH NIEHS","award":["U19 ES019525"],"award-info":[{"award-number":["U19 ES019525"]}]},{"name":"NIH NIEHS","award":["R01 ES016246"],"award-info":[{"award-number":["R01 ES016246"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2016,1,15]]},"abstract":"<jats:p>Pulmonary instillation of multiwalled carbon nanotubes (MWCNT) has the potential to promote cardiovascular derangements, but the mechanisms responsible are currently unclear. We hypothesized that exposure to MWCNT would result in increased epithelial barrier permeability by 24 h postexposure and initiate a signaling process involving IL-6\/gp130 transsignaling in peripheral vascular tissue. To test this hypothesis we assessed the impact of 1 and 10 \u03bcg\/cm<jats:sup>2<\/jats:sup>MWCNT on transepithelial electrical resistance (TEER) and expression of barrier proteins and cell activation in vitro using normal human bronchial epithelial primary cells. Parallel studies using male Sprague-Dawley rats instilled with 100 \u03bcg MWCNT measured bronchoalveolar lavage (BAL) differential cell counts, BAL fluid total protein, and lung water-to-tissue weight ratios 24 h postexposure and quantified serum concentrations of IL-6, soluble IL-6r, and soluble gp130. Aortic sections were examined immunohistochemically for gp130 expression, and gp130 mRNA\/protein expression was evaluated in rat lung, heart, and aortic tissue homogenates. Our in vitro findings indicate that 10 \u03bcg\/cm<jats:sup>2<\/jats:sup>MWCNT decreased the development of TEER and zonula occludens-1 expression relative to the vehicle. In rats MWCNT instillation increased BAL protein, lung water, and induced pulmonary eosinophilia. Serum concentrations of soluble gp130 decreased, aortic endothelial expression of gp130 increased, and expression of gp130 in the lung was downregulated in the MWCNT-exposed group. We propose that pulmonary exposure to MWCNT can manifest as a reduced epithelial barrier and activator of vascular gp130-associated transsignaling that may promote susceptibility to cardiovascular derangements.<\/jats:p>","DOI":"10.1152\/ajplung.00384.2014","type":"journal-article","created":{"date-parts":[[2015,11,21]],"date-time":"2015-11-21T03:10:48Z","timestamp":1448075448000},"page":"L142-L154","source":"Crossref","is-referenced-by-count":13,"title":["Pulmonary instillation of MWCNT increases lung permeability, decreases gp130 expression in the lungs, and initiates cardiovascular IL-6 transsignaling"],"prefix":"10.1152","volume":"310","author":[{"given":"Leslie C.","family":"Thompson","sequence":"first","affiliation":[{"name":"Department of Physiology, Brody School of Medicine at East Carolina University, Greenville, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan A.","family":"Holland","sequence":"additional","affiliation":[{"name":"Department of Physiology, Brody School of Medicine at East Carolina University, Greenville, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan J.","family":"Snyder","sequence":"additional","affiliation":[{"name":"NanoHealth Program, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, North Carolina; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Toxicology, Brody School of Medicine at East Carolina University, Greenville, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Becak","sequence":"additional","affiliation":[{"name":"Department of Physiology, Brody School of Medicine at East Carolina University, Greenville, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jillian T.","family":"Odom","sequence":"additional","affiliation":[{"name":"Department of Physiology, Brody School of Medicine at East Carolina University, Greenville, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin S.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Wake Forest University Institute of Regenerative Medicine, Winston-Salem, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared M.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Toxicology, Brody School of Medicine at East Carolina University, Greenville, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kymberly M.","family":"Gowdy","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Toxicology, Brody School of Medicine at East Carolina University, Greenville, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Wingard","sequence":"additional","affiliation":[{"name":"Department of Physiology, Brody School of Medicine at East Carolina University, Greenville, North Carolina;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107971200"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.127"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-015-0191-9"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kft328"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2010.01910.x"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3109\/08958370903365692"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.1002286"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-8-27"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.7938"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2009-0456OC"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000164237.58200.5A"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-9-17"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.226928"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1021\/tx200351d"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2010.08.019"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.2174\/157340308785160570"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-11-28"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0b013e32831e9948"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.01867.x"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-8-6"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201200873"},{"key":"B22","doi-asserted-by":"crossref","first-page":"4583","DOI":"10.4049\/jimmunol.163.8.4583","volume":"163","author":"Klouche M","year":"1999","journal-title":"J Immunol"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.3109\/17435390.2011.606926"},{"key":"B24","doi-asserted-by":"crossref","first-page":"2508","DOI":"10.1182\/blood.V91.7.2508","volume":"91","author":"Lacy P","year":"1998","journal-title":"Blood"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2012.02566.x"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1007\/s12012-009-9060-4"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2010.05.017"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2009.01.014"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1002\/wnan.1194"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E05-07-0650"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2004080628"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1007\/s12012-013-9213-3"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2009.01.017"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600106"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfp057"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2009.03.002"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1080\/15287390903486527"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.42.30041"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-119701"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2015.04.010"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-013-1116-3"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.4989"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.3109\/17435390.2014.918203"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2010.03741.x"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2009.305"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2013-0019OC"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00433.2010"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2013-0090OC"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.1.601"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0128888"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S65567"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-10-35"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0508946103"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.3390\/ijms131113781"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2010-0169OC"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.12.1927"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2014-0221OC"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.3109\/17435390.2012.744858"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfu008"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/7.2.214"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.02.032"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.043"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-9-38"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201203-0455PP"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2014.07.074"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.075952"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-9-35"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1126\/science.1967851"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1080\/15419060212185"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2008.02.011"}],"container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.00384.2014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,15]],"date-time":"2023-08-15T22:51:20Z","timestamp":1692139880000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.00384.2014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,15]]},"references-count":70,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,1,15]]}},"alternative-id":["10.1152\/ajplung.00384.2014"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.00384.2014","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,15]]}}}