{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:46:10Z","timestamp":1789771570525,"version":"4.0.1"},"reference-count":28,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["K08HL127075"],"award-info":[{"award-number":["K08HL127075"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL122895"],"award-info":[{"award-number":["R01HL122895"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL133751"],"award-info":[{"award-number":["R01HL133751"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R03HL155075-01A1"],"award-info":[{"award-number":["R03HL155075-01A1"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006806","name":"MUSC | Hollings Cancer Center, Medical University of South Carolina","doi-asserted-by":"publisher","award":["P30 CA138313"],"award-info":[{"award-number":["P30 CA138313"]}],"id":[{"id":"10.13039\/100006806","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:p> We previously showed that pericyte-like cells derived from the FoxD1-lineage contribute to myofibroblasts following bleomycin-induced lung injury. However, their functional significance in lung fibrosis remains unknown. In this study, we used a model of lung pericyte-like cell ablation to test the hypothesis that pericyte-like cell ablation attenuates lung fibrosis in bleomycin-induced lung injury. Lung fibrosis was induced by intratracheal instillation of bleomycin. To ablate pericyte-like cells in the lung, diphtheria toxin (DT) was administered to Foxd1-Cre;Rosa26-iDTR mice at two different phases of bleomycin-induced lung injury. For early ablation, we coadministered bleomycin with DT and harvested mice at days 7 and 21. To test the effect of ablation after acute injury, we delivered DT 7 days after bleomycin administration. We assessed fibrosis by lung hydroxyproline content and semiquantitative analysis of picrosirius red staining. We performed bronchoalveolar lavage to determine cell count and differential. We also interrogated mRNA expression of fibrosis-related genes in whole lung RNA. Compared with DT-insensitive littermates where pericyte-like cells were not ablated, DT-sensitive animals exhibited no difference in fibrosis at day 21 both in the early and late pericyte ablation models. However, early ablation of pericytes reduced acute lung inflammation, as indicated by decreased inflammatory cells. Our data confirm a role for pericytes in regulating pulmonary inflammation in early lung injury. <\/jats:p>","DOI":"10.1152\/ajplung.00392.2021","type":"journal-article","created":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T02:49:27Z","timestamp":1645670967000},"page":"L607-L616","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Effect of lung pericyte-like cell ablation on the bleomycin model of injury and repair"],"prefix":"10.1152","volume":"322","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6408-0773","authenticated-orcid":true,"given":"Chi F.","family":"Hung","sequence":"first","affiliation":[{"name":"Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, University of Washington, Seattle, Washington"},{"name":"Center for Lung Biology, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6692-0430","authenticated-orcid":true,"given":"Carole L.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Division of Allergy, Pulmonary, and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Hua","family":"Chow","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, University of Washington, Seattle, Washington"},{"name":"Center for Lung Biology, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne C.","family":"Liles","sequence":"additional","affiliation":[{"name":"Center for Lung Biology, University of Washington, Seattle, Washington"},{"name":"Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2480-4367","authenticated-orcid":true,"given":"Sina A.","family":"Gharib","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, University of Washington, Seattle, Washington"},{"name":"Center for Lung Biology, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8860-405X","authenticated-orcid":true,"given":"William A.","family":"Altemeier","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, University of Washington, Seattle, Washington"},{"name":"Center for Lung Biology, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9189-7567","authenticated-orcid":true,"given":"Lynn M.","family":"Schnapp","sequence":"additional","affiliation":[{"name":"Division of Allergy, Pulmonary, and Critical Care Medicine, Department of Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-10-103"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2008.02.012"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605669103"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00114.2014"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1117988108"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.07.034"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2013-204608"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1007\/s43152-020-00022-9"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.07.001"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00349.2016"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201903192RR"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201212-2297OC"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2016-0083MA"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn224"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks420"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.6.509"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5193(05)80548-X"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-14-102"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba1983"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1126\/science.1203165"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20111622"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00480"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2006.00192.x"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.06.028"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.90"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar2971"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2941-1"}],"container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajplung.00392.2021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T21:43:42Z","timestamp":1649281422000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajplung.00392.2021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,1]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4,1]]}},"alternative-id":["10.1152\/ajplung.00392.2021"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.00392.2021","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,1]]},"assertion":[{"value":"2021-09-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-13","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}