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Using fura-2, we studied the effects of quinpirole, a D2 receptor agonist, on type 1 cell [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>response to 90-s hypoxia challenges (Po<jats:sub>2<\/jats:sub>\u223c1\u20132 mmHg). Cells were sequentially exposed to the following challenges: 1) hypoxia control, 2) hypoxia plus quinpirole, and 3) hypoxia plus quinpirole plus sulpiride (D2 receptor antagonist). In the 11- to 16-day-old group, type 1 cell [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>increased \u223c3 to 4-fold over resting [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in response to hypoxia. Quinpirole (10 \u03bcM) significantly blunted the peak [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>response to hypoxia. Repeat challenge with hypoxia plus 10 \u03bcM quinpirole in the presence of 10 \u03bcM sulpiride partially restored the hypoxia [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>response. In sharp contrast to the older aged group, 10 \u03bcM quinpirole had minimal effect on hypoxia response of type 1 cells from 1-day-olds and a small but significant effect at 3 days of age. We conclude that stimulation of dopamine D2 receptors inhibits type 1 cell [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>response to hypoxia, consistent with an inhibitory autoreceptor role. These findings suggest dopamine-mediated inhibition and oxygen sensitivity increase with age on a similar time course and do not support a role for dopamine as a major mediator of carotid chemoreceptor resetting.<\/jats:p>","DOI":"10.1152\/ajplung.00414.2003","type":"journal-article","created":{"date-parts":[[2005,1,29]],"date-time":"2005-01-29T07:24:47Z","timestamp":1106983487000},"page":"L910-L916","source":"Crossref","is-referenced-by-count":29,"title":["Dopamine D2 receptor modulation of carotid body type 1 cell intracellular calcium in developing rats"],"prefix":"10.1152","volume":"288","author":[{"given":"J. L.","family":"Carroll","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. M.","family":"Boyle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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