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We focused on specific Ca<jats:sup>2+<\/jats:sup> regulation- and inflammation-related signaling cascades and quantified BDNF secretion. We find that TNF-\u03b1 enhances BDNF release by ASM cells, via several mechanisms relevant to asthma, including transient receptor potential channels TRPC3 and TRPC6 (but not TRPC1), ERK 1\/2, PI3K, PLC, and PKC cascades, Rho kinase, and transcription factors cAMP response element binding protein and nuclear factor of activated T cells. Basal BDNF expression and secretion are elevated in asthmatic ASM and increase further with TNF-\u03b1 exposure, involving many of these regulatory mechanisms. We conclude that airway BDNF secretion is regulated at multiple levels, providing a basis for autocrine effects of BDNF under conditions of inflammation and disease, with potential downstream influences on contractility and remodeling. <\/jats:p>","DOI":"10.1152\/ajplung.00414.2015","type":"journal-article","created":{"date-parts":[[2016,6,18]],"date-time":"2016-06-18T02:44:02Z","timestamp":1466217842000},"page":"L270-L279","source":"Crossref","is-referenced-by-count":31,"title":["Mechanisms of BDNF regulation in asthmatic airway smooth muscle"],"prefix":"10.1152","volume":"311","author":[{"given":"Bharathi","family":"Aravamudan","sequence":"first","affiliation":[{"name":"Departments of Anesthesiology, Mayo Clinic, Rochester, Minnesota; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Departments of Anesthesiology, Mayo Clinic, Rochester, Minnesota; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina M.","family":"Pabelick","sequence":"additional","affiliation":[{"name":"Departments of Anesthesiology, Mayo Clinic, Rochester, Minnesota; and"},{"name":"Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. 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