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We tested these hypotheses using small interfering RNA (siRNA) directed against the erbB4 gene to silence erbB4 receptor function in cultures of primary day 19 fetal rat lung type II cells. ErbB4 siRNA treatment inhibited erbB4 receptor protein expression, fibroblast-conditioned medium induced erbB4 phosphorylation, and fetal surfactant phospholipid synthesis. Cell proliferation, measured as thymidine incorporation, was also inhibited by erbB4 siRNA treatment. Downregulation of erbB4 receptor protein changed erbB1 localization at baseline and after stimulation, as determined by confocal microscopy and subcellular fractionation. We conclude that erbB4 is an important receptor in the control of fetal lung type II cell maturation.<\/jats:p>","DOI":"10.1152\/ajplung.00451.2006","type":"journal-article","created":{"date-parts":[[2007,6,2]],"date-time":"2007-06-02T01:18:27Z","timestamp":1180747107000},"page":"L429-L435","source":"Crossref","is-referenced-by-count":28,"title":["ErbB4 regulates fetal surfactant phospholipid synthesis in primary fetal rat type II cells"],"prefix":"10.1152","volume":"293","author":[{"given":"Katja","family":"Zscheppang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Washa","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MaryAnn V.","family":"Volpe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heber C.","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane E. 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