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Incubation with intact Pc organisms also did not impair dynamic surface tension lowering in suspensions of WLS or centrifuged large surfactant aggregates on a bubble surfactometer (37\u00b0C, 20 cycles\/min, 0.5 and 2.5 mg phospholipid\/ml). However, exposure of WLS or CLSE to disrupted (sonicated) Pc organisms led to severe detriments in activity, with minimum surface tensions of 17\u201319 mN\/m vs. &lt;1 mN\/m for surfactants alone. Extracted hydrophobic chemical components from Pc (98.8% lipids, 0.1 mM) reduced the surface activity of WLS and CLSE similarly to sonicated Pc organisms, whereas extracted hydrophilic chemical components from Pc (primarily proteins) had only minor effects on surface tension lowering. These results indicate that in addition to surfactant dysfunction induced by inflammatory lung injury and edema-derived inhibitors in Pc pneumonia, disrupted Pc organisms in the alveolar lumen also have the potential to directly inhibit endogenous and exogenous lung surfactants in affected patients.<\/jats:p>","DOI":"10.1152\/ajplung.00453.2004","type":"journal-article","created":{"date-parts":[[2005,5,10]],"date-time":"2005-05-10T22:48:51Z","timestamp":1115765331000},"page":"L1124-L1131","source":"Crossref","is-referenced-by-count":10,"title":["Inhibition of surfactant activity by<i>Pneumocystis carinii<\/i>organisms and components in 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