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Previously, others and we have reported that, similar to cigarette smoke, e-cig aerosols decrease CFTR-mediated ion transport across airway epithelium. However, it is unclear whether such defective epithelial ion transport by e-cig aerosols occurs in vivo and what the singular contribution of inhaled nicotine is to impairments in mucociliary clearance (MCC), the primary physiologic defense of the airways. Here, we tested the effects of nicotine aerosols from e-cigs in primary human bronchial epithelial (HBE) cells and two animal models, rats and ferrets, known for their increasing physiologic complexity and potential for clinical translation, followed by in vitro and in vivo electrophysiologic assays for CFTR activity and micro-optical coherence tomography (\u03bcOCT) image analyses for alterations in airway mucus physiology. Data presented in this report indicate nicotine in e-cig aerosols causes 1) reduced CFTR and epithelial Na<jats:sup>+<\/jats:sup> channel (ENaC)-mediated ion transport, 2) delayed MCC, and 3) diminished airway surface hydration, as determined by periciliary liquid depth analysis. Interestingly, the common e-cig vehicles vegetable glycerin and propylene glycol did not affect CFTR function or MCC in vivo despite their significant adverse effects in vitro. Overall, our studies contribute to an improved understanding of inhaled nicotine effects on lung health among e-cig users and inform pathologic mechanisms involved in altered host defense and increased risk for tobacco-associated lung diseases. <\/jats:p>","DOI":"10.1152\/ajplung.00453.2021","type":"journal-article","created":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T13:01:10Z","timestamp":1677589270000},"page":"L557-L570","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":24,"title":["Nicotine aerosols diminish airway CFTR function and mucociliary clearance"],"prefix":"10.1152","volume":"324","author":[{"given":"Lawrence W.","family":"Rasmussen","sequence":"first","affiliation":[{"name":"Department of Medicine, The University of Alabama at Birmingham, Birmingham, Alabama, United States"},{"name":"Department of Environmental Health Sciences, The University of Alabama at Birmingham, Birmingham, Alabama, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Stanford","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Alabama at Birmingham, Birmingham, Alabama, United States"},{"name":"Cystic Fibrosis Research Center, The University of Alabama at Birmingham, Birmingham, Alabama, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"LaFontaine","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Alabama at Birmingham, Birmingham, Alabama, United States"},{"name":"Cystic Fibrosis Research Center, The University of Alabama at Birmingham, Birmingham, Alabama, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Demarcus","family":"Allen","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Alabama at Birmingham, Birmingham, Alabama, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3947-4871","authenticated-orcid":false,"given":"S. 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