{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T03:54:34Z","timestamp":1793332474798,"version":"4.5.0"},"reference-count":49,"publisher":"American Physiological Society","issue":"1","funder":[{"name":"ADRN","award":["1\/2017-2018"],"award-info":[{"award-number":["1\/2017-2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"<jats:p>Secreted phospholipase A<jats:sub>2<\/jats:sub>hydrolyzes surfactant phospholipids and is crucial for the inflammatory cascade; preterm neonates are treated with exogenous surfactant, but the interaction between surfactant and phospholipase is unknown. We hypothesize that this interplay is complex and the enzyme plays a relevant role in neonates needing surfactant replacement. We aimed to: 1) identify phospholipases A<jats:sub>2<\/jats:sub>isoforms expressed in preterm lung; 2) study the enzyme role on surfactant retreatment and function and the effect of exogenous surfactant on the enzyme system; and 3) verify whether phospholipase A<jats:sub>2<\/jats:sub>is linked to respiratory outcomes. In bronchoalveolar lavages of preterm neonates, we measured enzyme activity (alone or with inhibitors), enzyme subtypes, surfactant protein-A, and inflammatory mediators. Surfactant function and phospholipid profile were also tested. Urea ratio was used to obtain epithelial lining fluid concentrations. Follow-up data were prospectively collected. Subtype-IIA is the main phospholipase isoform in preterm lung, although subtype-IB may be significantly expressed. Neonates needing surfactant retreatment have higher enzyme activity ( P = 0.021) and inflammatory mediators ( P always \u2264 0.001) and lower amounts of phospholipids ( P always &lt; 0.05). Enzyme activity was inversely correlated to surfactant adsorption (\u03c1\u2009=\u2009\u22120.6; P = 0.008; adjusted P = 0.009), total phospholipids (\u03c1\u2009=\u2009\u22120.475; P = 0.05), and phosphatidylcholine (\u03c1\u2009=\u2009\u22120.622; P = 0.017). Exogenous surfactant significantly reduced global phospholipase activity ( P &lt; 0.001) and subtype-IIA ( P = 0.005) and increased dioleoylphosphatidylglycerol ( P &lt; 0.001) and surfactant adsorption ( P &lt; 0.001). Enzyme activity correlated with duration of ventilation (\u03c1\u2009=\u20090.679, P = 0.005; adjusted P = 0.04) and respiratory morbidity score at 12 mo postnatal age (\u03c4<jats:sub>-b<\/jats:sub>\u2009=\u20090.349, P = 0.037; adjusted P = 0.043) but was not associated with mortality, bronchopulmonary dysplasia, or other long-term respiratory outcomes.<\/jats:p>","DOI":"10.1152\/ajplung.00462.2019","type":"journal-article","created":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T20:17:02Z","timestamp":1589401022000},"page":"L95-L104","source":"Crossref","is-referenced-by-count":21,"title":["Surfactant-secreted phospholipase A<sub>2<\/sub>interplay and respiratory outcome in preterm neonates"],"prefix":"10.1152","volume":"319","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3846-4834","authenticated-orcid":false,"given":"Daniele","family":"De Luca","sequence":"first","affiliation":[{"name":"Division of Pediatrics and Neonatal Critical Care, \u201cA.B\u00e9cl\u00e8re\u201d Medical Center, South Paris University Hospitals, Assistance Publique \u2013 H\u00f4pitaux de Paris (APHP), Paris, France"},{"name":"Physiopathology and Therapeutic Innovation Unit-INSERM U999, South Paris-Saclay University, Paris, France"},{"name":"Cystic fibrosis and Bronchial diseases team-INSERM U938, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivani","family":"Shankar-Aguilera","sequence":"additional","affiliation":[{"name":"Division of Pediatrics and Neonatal Critical Care, \u201cA.B\u00e9cl\u00e8re\u201d Medical Center, South Paris University Hospitals, Assistance Publique \u2013 H\u00f4pitaux de Paris (APHP), Paris, France"},{"name":"Cystic fibrosis and Bronchial diseases team-INSERM U938, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Autilio","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Biology, and Research Institut-Hospital \u201c12 de Octubre,\u201d Complutense University, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Raschetti","sequence":"additional","affiliation":[{"name":"Division of Pediatrics and Neonatal Critical Care, \u201cA.B\u00e9cl\u00e8re\u201d Medical Center, South Paris University Hospitals, Assistance Publique \u2013 H\u00f4pitaux de Paris (APHP), Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Vedovelli","sequence":"additional","affiliation":[{"name":"PCare Laboratory, Fondazione Istituto di Ricerca Pediatrica \u201cCitt\u00e0 della Speranza,\u201d Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Fitting","sequence":"additional","affiliation":[{"name":"Cytokines and Inflammation Unit, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Payr\u00e9","sequence":"additional","affiliation":[{"name":"Universit\u00e9 C\u00f4te d\u2019Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Mol\u00e9culaire et Cellulaire, UMR7275, Valbonne Sophia Antipolis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Jeammet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 C\u00f4te d\u2019Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Mol\u00e9culaire et Cellulaire, UMR7275, Valbonne Sophia Antipolis, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesus","family":"Perez-Gil","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Biology, and Research Institut-Hospital \u201c12 de Octubre,\u201d Complutense University, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola E.","family":"Cogo","sequence":"additional","affiliation":[{"name":"PCare Laboratory, Fondazione Istituto di Ricerca Pediatrica \u201cCitt\u00e0 della Speranza,\u201d Padua, Italy"},{"name":"Division of Pediatrics, Department of Medicine and Surgery, University of Udine, Udine, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virgilio P.","family":"Carnielli","sequence":"additional","affiliation":[{"name":"PCare Laboratory, Fondazione Istituto di Ricerca Pediatrica \u201cCitt\u00e0 della Speranza,\u201d Padua, Italy"},{"name":"Division of Neonatology, \u201cG. 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