{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:39:06Z","timestamp":1790285946245,"version":"4.1.0"},"reference-count":49,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI130528"],"award-info":[{"award-number":["AI130528"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017540","name":"HHS | NIH | NHLBI | NHLBI Division of Intramural Research","doi-asserted-by":"publisher","award":["HL134625"],"award-info":[{"award-number":["HL134625"]}],"id":[{"id":"10.13039\/100017540","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017540","name":"HHS | NIH | NHLBI | NHLBI Division of Intramural Research","doi-asserted-by":"publisher","award":["HL123965"],"award-info":[{"award-number":["HL123965"]}],"id":[{"id":"10.13039\/100017540","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL123965"],"award-info":[{"award-number":["HL123965"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM124911"],"award-info":[{"award-number":["GM124911"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM103652"],"award-info":[{"award-number":["GM103652"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2022,10,1]]},"abstract":"<jats:p>Atrial natriuretic peptide (ANP) protects against acute lung injury (ALI), but the receptor that mediates this effect is not known. Transgenic mice with 0 (knockout), 1 (heterozygote), or 2 (wild-type) functional copies of Npr3, the gene that encodes for natriuretic peptide receptor-C (NPR-C), were treated with intravenous infusion of ANP or saline vehicle before oropharyngeal aspiration of Pseudomonas aeruginosa (PA103) or saline vehicle. Lung injury was assessed 4 h following aspiration by measurement of lung wet\/dry (W\/D) weight, whole lung leukocyte and cytokine levels, and protein, leukocyte, and cytokine concentration in bronchoalveolar lavage fluid (BALF). PA103 induced acute lung injury as evidenced by increases in lung W\/D ratio and protein concentration in BALF. The severity of PA103-induced lung injury did not differ between NPR-C genotypes. Treatment with intravenous ANP infusion reduced PA103-induced increases in lung W\/D and BALF protein concentration in all three NPRC genotypes. PA103 increased the percentage of leukocytes that were neutrophils and cytokine levels in whole lung and BALF in NPR-C wild-type and knockout mice. This effect was blunted by ANP in wild-type mice but not in the NPR-C knockout mice. NPR-C does not mediate the protective effect of ANP on endothelial cell permeability in settings of PA103-induced injury but may mediate the effect of ANP on inhibition of the recruitment of neutrophils to the lung and thereby attenuate the release of inflammatory cytokines.<\/jats:p>","DOI":"10.1152\/ajplung.00477.2021","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T12:01:04Z","timestamp":1660046464000},"page":"L438-L449","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Natriuretic peptide receptor-C mediates the inhibitory effect of atrial natriuretic peptide on neutrophil recruitment to the lung during acute lung injury"],"prefix":"10.1152","volume":"323","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3207-5301","authenticated-orcid":false,"given":"Elizabeth O.","family":"Harrington","sequence":"first","affiliation":[{"name":"Vascular Research Lab, Providence Veterans Administration Medical Center, Providence, Rhode Island"},{"name":"Division of Pulmonary, Sleep and Critical Care Medicine, Rhode Island Hospital, Providence, Rhode Island"},{"name":"Warren Alpert Medical School of Brown University, Providence, Rhode Island"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok","family":"Kumar","sequence":"additional","affiliation":[{"name":"Vascular Research Lab, Providence Veterans Administration Medical Center, Providence, Rhode Island"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7494-1055","authenticated-orcid":false,"given":"Verida","family":"Leandre","sequence":"additional","affiliation":[{"name":"Pathobiology Graduate Program, Brown University, Providence, Rhode Island"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary S.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Pathobiology Graduate Program, Brown University, Providence, Rhode Island"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brianna","family":"Guarino","sequence":"additional","affiliation":[{"name":"Vascular Research Lab, Providence Veterans Administration Medical Center, Providence, Rhode Island"},{"name":"Division of Pulmonary, Sleep and Critical Care Medicine, Rhode Island Hospital, Providence, Rhode Island"},{"name":"Warren Alpert Medical School of Brown University, Providence, Rhode Island"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Braza","sequence":"additional","affiliation":[{"name":"Vascular Research Lab, Providence Veterans Administration Medical Center, Providence, Rhode Island"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig T.","family":"Lefort","sequence":"additional","affiliation":[{"name":"Division of Surgical Research, Department of Surgery, Rhode Island Hospital, Providence, Rhode Island"},{"name":"Warren Alpert Medical School of Brown University, Providence, Rhode Island"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2997-5955","authenticated-orcid":false,"given":"James R.","family":"Klinger","sequence":"additional","affiliation":[{"name":"Vascular Research Lab, Providence Veterans Administration Medical Center, Providence, Rhode Island"},{"name":"Division of Pulmonary, Sleep and Critical Care Medicine, Rhode Island Hospital, Providence, Rhode Island"},{"name":"Warren Alpert Medical School of Brown University, Providence, Rhode Island"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-012-2682-1"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa050333"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-016-4571-5"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1159\/000045836"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-020-03240-7"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(20)30079-5"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2100433"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2021436"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-011110-130158"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2009-0210ST"},{"key":"B11","doi-asserted-by":"crossref","first-page":"3439","DOI":"10.4049\/jimmunol.158.7.3439","volume":"158","author":"Shanley TP","year":"1997","journal-title":"J Immunol"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2011-0392TR"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.13.7403"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1186\/1756-8722-6-61"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.76.2.8432776"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-70-3-582"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23360"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2005.11.001"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01249.2011"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2004.09.023"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1126\/science.1672777"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00508"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1084\/jem.171.5.1797"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.21.10542"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.2.612"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3233\/BIR-15067"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2004.08.034"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2018.08.031"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20010129"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12992"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1007\/BF01487743"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-2999(96)00859-x"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115752"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1210\/endo.143.3.8680"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.22.13444"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.1.175"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/s0167-0115(01)00218-x"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1155\/S0962935192000632"},{"key":"B39","doi-asserted-by":"crossref","first-page":"747","DOI":"10.3760\/cma.j.issn.0366-6999.20122435","volume":"126","author":"Zhu YB","year":"2013","journal-title":"Chin Med J (Engl)"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2014.11.023"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.046755-0"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01400.x"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504961102"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1186\/ar1939"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00202.2009"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00284.2010"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200101000-00019"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1378\/chest.114.1.223"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(90)90087-h"}],"container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajplung.00477.2021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T01:26:07Z","timestamp":1727745967000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajplung.00477.2021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,1]]},"references-count":49,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,10,1]]}},"alternative-id":["10.1152\/ajplung.00477.2021"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.00477.2021","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,1]]},"assertion":[{"value":"2021-11-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-02","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}