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To address normal murine airway mucociliary clearance, Alcian blue-stained mucus transport was measured ex vivo on tracheal tissues of na\u00efve C57BL\/6, Muc5b\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    , Muc5ac\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    , and EGFP-tagged Muc5b reporter mice. Close to the larynx with a few submucosal glands, the mucus appeared as thick bundles. More distally in the trachea and in large bronchi, Alcian blue-stained mucus was organized in cloud-like formations based on the Muc5b mucin. On tilted tissue, the mucus clouds moved upward toward the larynx with an average velocity of 12 \u00b5m\/s compared with 20 \u00b5m\/s for beads not associated with clouds. In Muc5ac\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice, Muc5b formed mucus strands attached to the tissue surface, while in Muc5b\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice, Muc5ac had a more variable appearance. The normal mouse lung mucus thus appears as discontinuous clouds, clearly different from the stagnant mucus layer in diseased lungs.\n                  <\/jats:p>","DOI":"10.1152\/ajplung.00485.2019","type":"journal-article","created":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T18:10:51Z","timestamp":1588183851000},"page":"L1270-L1279","source":"Crossref","is-referenced-by-count":29,"title":["Normal murine respiratory tract has its mucus concentrated in clouds based on the Muc5b mucin"],"prefix":"10.1152","volume":"318","author":[{"given":"Dalia","family":"Fakih","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, University of Gothenburg, Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Rodriguez-Pi\u00f1eiro","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, University of Gothenburg, Gothenburg, 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