{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:36:43Z","timestamp":1791121003657,"version":"4.1.0"},"reference-count":31,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:p>Endogenous hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) is naturally synthesized in various types of mammalian cells from l-cysteine in a reaction catalyzed by two enzymes, cystathionine-\u03b3-lyase (CSE) and\/or cystathionine-\u03b2-synthase. The latest studies have implied that H<jats:sub>2<\/jats:sub>S functions as a vasodilator and neurotransmitter. However, so far there is little information about the role played by H<jats:sub>2<\/jats:sub>S in systemic inflammation such as sepsis. Thus the aim of this study was to investigate the potential role of endogenous H<jats:sub>2<\/jats:sub>S in cecal ligation and puncture (CLP)-induced sepsis. Male Swiss mice were subjected to CLP-induced sepsis and treated with saline (ip), dl-propargylglycine (PAG, 50 mg\/kg ip), a CSE inhibitor, or sodium hydrosulfide (NaHS; 10 mg\/kg ip). PAG was administered either 1 h before or 1 h after the induction of sepsis, whereas NaHS was given at the same time of CLP. CLP-induced sepsis significantly increased the plasma H<jats:sub>2<\/jats:sub>S level and the liver H<jats:sub>2<\/jats:sub>S synthesis 8 h after CLP compared with sham operation. Induction of sepsis also resulted in a significant upregulation of CSE mRNA in liver. On the other hand, prophylactic as well as therapeutic administration of PAG significantly reduced sepsis-associated systemic inflammation, as evidenced by myeloperoxidase activity and histological changes in lung and liver, and attenuated the mortality of CLP-induced sepsis. Injection of NaHS significantly aggravated sepsis-associated systemic inflammation. Therefore, the effect of inhibition of H<jats:sub>2<\/jats:sub>S formation and administration of NaHS suggests that H<jats:sub>2<\/jats:sub>S plays a proinflammatory role in regulating the severity of sepsis and associated organ injury.<\/jats:p>","DOI":"10.1152\/ajplung.00489.2005","type":"journal-article","created":{"date-parts":[[2006,1,21]],"date-time":"2006-01-21T01:32:57Z","timestamp":1137807177000},"page":"L1193-L1201","source":"Crossref","is-referenced-by-count":155,"title":["Role of hydrogen sulfide in cecal ligation and puncture-induced sepsis in the mouse"],"prefix":"10.1152","volume":"290","author":[{"given":"Huili","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip 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