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Accordingly, we studied the effect of NO generated from S-nitroso- N-acetylpenicillamide (SNAP) and compared the effects of the nonselective inhibitor N<jats:sup>\u03c9<\/jats:sup>-nitro-l-arginine methyl ester (l-NAME) and the selective nNOS inhibitor 1-(2-trifluoromethylphenyl)-imidazole (TRIM) on chemosensory dose-response curves induced by nicotine and NaCN and responses to hypoxia (Po<jats:sub>2<\/jats:sub>\u2248 30 Torr). CBs excised from pentobarbitone-anesthetized cats were perfused in vitro with Tyrode at 38\u00b0C and pH 7.40, and chemosensory discharges were recorded from the carotid sinus nerve. SNAP (100 \u03bcM) reduced the responses to nicotine and NaCN. l-NAME (1 mM) enhanced the responses to nicotine and NaCN by increasing their duration, but TRIM (100 \u03bcM) only enhanced the responses to high doses of NaCN. The amplitude of the response to hypoxia was enhanced by l-NAME but not by TRIM. Our results suggest that both isoforms contribute to the NO action, but eNOS being the main source for NO in the cat CB and exerting a tonic effect upon chemoreceptor activity.<\/jats:p>","DOI":"10.1152\/ajplung.00494.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:19:54Z","timestamp":1425413994000},"page":"L57-L68","source":"Crossref","is-referenced-by-count":33,"title":["Inhibitory effects of NO on carotid body: contribution of neural and endothelial nitric oxide synthase isoforms"],"prefix":"10.1152","volume":"284","author":[{"given":"Viviana","family":"Vald\u00e9s","sequence":"first","affiliation":[{"name":"Laboratorio de Neurobiolog\u0131\u0301a, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 1, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u00edas","family":"Mosqueira","sequence":"additional","affiliation":[{"name":"Laboratorio de Neurobiolog\u0131\u0301a, 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