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Some patients with ADPKD experience extrarenal manifestations, including radiologic and clinical bronchiectasis. We hypothesized that TRPP2 may regulate airway smooth muscle (ASM) tension. Thus, we used smooth muscle- Pkd2 conditional knockout ( Pkd2<jats:sup>SM-CKO<\/jats:sup>) mice to investigate whether TRPP2 regulated ASM tension and whether TRPP2 deficiency contributed to bronchiectasis associated with ADPKD. Compared with wild-type mice, Pkd2<jats:sup>SM-CKO<\/jats:sup>mice breathed more shallowly and faster, and their cross-sectional area ratio of bronchi to accompanying pulmonary arteries was higher, suggesting that TRPP2 may regulate ASM tension and contribute to the occurrence of bronchiectasis in ADPKD. In a bioassay examining isolated tracheal ring tension, no significant difference was found for high-potassium-induced depolarization of the ASM between the two groups, indicating that TRPP2 does not regulate depolarization-induced ASM contraction. By contrast, carbachol-induced contraction of the ASM derived from Pkd2<jats:sup>SM-CKO<\/jats:sup>mice was significantly reduced compared with that in wild-type mice. In addition, relaxation of the carbachol-precontracted ASM by isoprenaline, a \u03b2-adrenergic receptor agonist that acts through the cAMP\/adenylyl cyclase pathway, was also significantly attenuated in Pkd2<jats:sup>SM-CKO<\/jats:sup>mice compared with that in wild-type mice. Thus, TRPP2 deficiency suppressed both contraction and relaxation of the ASM. These results provide a potential target for regulating ASM tension and for developing therapeutic alternatives for some ADPKD complications of the respiratory system or for independent respiratory disease, especially bronchiectasis.<\/jats:p>","DOI":"10.1152\/ajplung.00513.2018","type":"journal-article","created":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T11:15:42Z","timestamp":1565781342000},"page":"L466-L474","source":"Crossref","is-referenced-by-count":11,"title":["Role of TRPP2 in mouse airway smooth muscle tension and respiration"],"prefix":"10.1152","volume":"317","author":[{"given":"Dacheng","family":"Sang","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences, Anhui Medical University, Hefei, China"},{"name":"Department of Orthopedic Surgery, Beijing TianTan Hospital, Capital Medical University, Beijing, 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