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Previous studies described barrier-enhancing and anti-inflammatory effects of PGE<jats:sub>2<\/jats:sub>and PGI<jats:sub>2<\/jats:sub>on vascular endothelial cells (EC). Yet, the effects of other PG members on EC barrier and inflammatory activation have not been systematically analyzed. This study compared effects of PGE<jats:sub>2<\/jats:sub>, PGI<jats:sub>2<\/jats:sub>, PGF<jats:sub>2\u03b1<\/jats:sub>, PGA<jats:sub>2<\/jats:sub>, PGJ<jats:sub>2<\/jats:sub>, and PGD<jats:sub>2<\/jats:sub>on human pulmonary EC. EC permeability was assessed by measurements of transendothelial electrical resistance and cell monolayer permeability for FITC-labeled tracer. Anti-inflammatory effects of PGs were evaluated by analysis of expression of adhesion molecule ICAM1 and secretion of soluble ICAM1 and cytokines by EC. PGE<jats:sub>2<\/jats:sub>, PGI<jats:sub>2<\/jats:sub>, and PGA<jats:sub>2<\/jats:sub>exhibited the most potent barrier-enhancing effects and most efficient attenuation of thrombin-induced EC permeability and contractile response, whereas PGI<jats:sub>2<\/jats:sub>effectively suppressed thrombin-induced permeability but was less efficient in the attenuation of prolonged EC hyperpermeability caused by interleukin-6 or bacterial wall lipopolysaccharide, LPS. PGD<jats:sub>2<\/jats:sub>showed a modest protective effect on the EC inflammatory response, whereas PGF<jats:sub>2\u03b1<\/jats:sub>and PGJ<jats:sub>2<\/jats:sub>were without effect on agonist-induced EC barrier dysfunction. In vivo, PGE<jats:sub>2<\/jats:sub>, PGI<jats:sub>2<\/jats:sub>, and PGA<jats:sub>2<\/jats:sub>attenuated LPS-induced lung inflammation, whereas PGF<jats:sub>2\u03b1<\/jats:sub>and PGJ<jats:sub>2<\/jats:sub>were without effect. Interestingly, PGD<jats:sub>2<\/jats:sub>exhibited a protective effect in the in vivo model of LPS-induced lung injury. This study provides a comprehensive analysis of barrier-protective and anti-inflammatory effects of different prostaglandins on lung EC in vitro and in vivo and identifies PGE<jats:sub>2<\/jats:sub>, PGI<jats:sub>2<\/jats:sub>, and PGA<jats:sub>2<\/jats:sub>as prostaglandins with the most potent protective properties.<\/jats:p>","DOI":"10.1152\/ajplung.00519.2016","type":"journal-article","created":{"date-parts":[[2017,6,30]],"date-time":"2017-06-30T00:20:28Z","timestamp":1498782028000},"page":"L710-L721","source":"Crossref","is-referenced-by-count":26,"title":["Effects of prostaglandin lipid mediators on agonist-induced lung endothelial permeability and inflammation"],"prefix":"10.1152","volume":"313","author":[{"given":"Yunbo","family":"Ke","sequence":"first","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Illinois;"},{"name":"Department of Anesthesiology, University of Maryland School of Medicine, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga V.","family":"Oskolkova","sequence":"additional","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Illinois;"},{"name":"Department of Pharmaceutical Chemistry, University of Graz, Graz, Austria;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolene","family":"Sarich","sequence":"additional","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Illinois;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Tian","sequence":"additional","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Illinois;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Sitikov","sequence":"additional","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Illinois;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohan E.","family":"Tulapurkar","sequence":"additional","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, University of Chicago, Chicago, Illinois;"},{"name":"Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland; 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