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We report lower expression of glutamate receptor NMDA-type subunit 2B (GluN2B), a subunit composing NMDARs expected to affect cell survival\/proliferation of pulmonary artery smooth muscle cells (PASMCs), in PAH patient lungs. PASMC exposure to PDGF-BB stimulated immediate increased levels of phosphorylated Src family kinases (SFKs) together with increased phosphorylated GluN2B (its active form) and cell surface relocalization, suggesting a cross talk between PDGFR-recruited SFKs and NMDAR. Selective inhibition of PDGFR-\u03b2 or SFKs with imatinib or A-419259, respectively, on one hand, or with specific small-interfering RNAs (siRNAs) on the other hand, aborted PDGF-induced phosphorylation of GluN2B, thus validating the pathway. Selective inhibition of GluN2B using R\u00f625-6981 and silencing with specific siRNA, in the presence of PDGF-BB, significantly increased both migration and proliferation of PASMCs, thus strengthening the functional importance of the pathway. Together, these results indicate that GluN2B-type NMDAR activation may confer to PASMCs antiproliferative and antimigratory properties. The decreased levels of GluN2B observed in PAH pulmonary arteries could mediate the excessive proliferation of PASMCs, thus contributing to medial hyperplasia and PAH development. <\/jats:p>","DOI":"10.1152\/ajplung.00537.2017","type":"journal-article","created":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T11:14:20Z","timestamp":1544699660000},"page":"L445-L455","source":"Crossref","is-referenced-by-count":20,"title":["Functional interaction between PDGF\u03b2 and GluN2B-containing NMDA receptors in smooth muscle cell proliferation and migration in pulmonary arterial hypertension"],"prefix":"10.1152","volume":"316","author":[{"given":"Marceau","family":"Quatredeniers","sequence":"first","affiliation":[{"name":"Inserm UMR_S 999, H\u00f4pital Marie Lannelongue, Le Plessis-Robinson, France"},{"name":"Universit\u00e9 Paris-Sud, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Paris-Saclay, Le Kremlin-Bic\u00eatre, 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