{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T15:02:44Z","timestamp":1785855764892,"version":"3.56.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1990,6,1]]},"abstract":"<jats:p> In airway epithelia, adenosine 3',5'-cyclic monophosphate (cAMP) stimulates Cl- secretion by activating apical membrane Cl- channels and basolateral membrane K+ channels. Cl- channels are regulated by cAMP-dependent phosphorylation, whereas K+ channels are regulated by the cytosolic Ca2+ concentration, [Ca2+]c. Our recent observation that cAMP increases [Ca2+]c suggested that cAMP might indirectly regulate K+ channels by increasing [Ca2+]c. To study regulation of K+ channels we measured 86Rb efflux, single K+ channels in membrane patches, and [Ca2+]c with the fluorescent indicator fura-2. Isoproterenol and Ca2+ ionophore, A23187, transiently increased [Ca2+]c and transiently stimulated 86Rb efflux. Stimulation of 86Rb efflux resulted from release of intracellular Ca2+ stores. 86Rb efflux was blocked by Ba2+ or charybdotoxin, but not by tetraethylammonium. Charybdotoxin prevented all of the 86Rb efflux that was stimulated by A23187 or by forskolin. Charybdotoxin also blocked the low-conductance inwardly rectifying K+ channel (KCLIC) in membrane patches. These results indicate that the KCLIC channel is responsible for the Ca2(+)-dependent increase in K+ permeability in airway epithelial cells. They also indicate that cAMP-induced release of intracellular Ca2+ is sufficient to activate K+ channels. <\/jats:p>","DOI":"10.1152\/ajplung.1990.258.6.l334","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T01:42:41Z","timestamp":1513993361000},"page":"L334-L342","source":"Crossref","is-referenced-by-count":13,"title":["Basolateral K+ channels in airway epithelia. I. Regulation by Ca2+ and block by charybdotoxin"],"prefix":"10.1152","volume":"258","author":[{"given":"J. D.","family":"McCann","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Physiology, University of Iowa, College of Medicine, Iowa City 52242."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Physiology, University of Iowa, College of Medicine, Iowa City 52242."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Physiology, University of Iowa, College of Medicine, Iowa City 52242."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Kaczorowski","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Physiology, University of Iowa, College of Medicine, Iowa City 52242."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J.","family":"Welsh","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Physiology, University of Iowa, College of Medicine, Iowa City 52242."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1990.258.6.L334","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:06:16Z","timestamp":1567958776000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1990.258.6.L334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1990,6,1]]}},"alternative-id":["10.1152\/ajplung.1990.258.6.L334"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1990.258.6.l334","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6,1]]}}}