{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T02:42:30Z","timestamp":1761964950114},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1990,6,1]]},"abstract":"<jats:p> We previously described a Ca2(+)-activated K+ channel (KCLIC) in airway epithelial cells [J. D. McCann, J. Matsuda, M. Garcia, G. Kaczorowski, and M. J. Welsh. Am. J. Physiol 258 (Lung Cell. Mol. Physiol. 2): L334-L342, 1990]. To determine whether the KCLIC channel is a basolateral membrane channel and to understand its role in Cl- secretion, we studied airway epithelial cells grown on permeable supports. When cells were stimulated with A23187, charybdotoxin (ChTX) inhibited Cl- secretion and 86Rb efflux at the same concentrations, indicating that the KCLIC channel is required for Ca2(+)-stimulated Cl- secretion. We also investigated the function of K+ channels in adenosine 3',5'-cyclic monophosphate-stimulated secretion. Addition of isoproterenol caused a biphasic increase in Cl- secretion; the time course of the transient component correlated with the time course of the isoproterenol-induced increase in Ca2+ concentration [( Ca2+]c). ChTX inhibited the transient component, but not the prolonged component of secretion; Ba2+ inhibited the sustained component. These results suggest that when cells are grown on permeable supports isoproterenol-induced secretion depends on activation of two types of K+ channel: the KCLIC channel that is stimulated initially and a ChTX-insensitive K+ channel that is stimulated during sustained secretion. This conclusion was supported by measurement of 86Rb efflux from cell monolayers <\/jats:p>","DOI":"10.1152\/ajplung.1990.258.6.l343","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T01:42:41Z","timestamp":1513993361000},"page":"L343-L348","source":"Crossref","is-referenced-by-count":15,"title":["Basolateral K+ channels in airway epithelia. II. Role in Cl- secretion and evidence for two types of K+ channel"],"prefix":"10.1152","volume":"258","author":[{"given":"J. D.","family":"McCann","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Physiology, University of Iowa College of Medicine, Iowa City 52242."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Welsh","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Physiology, University of Iowa College of Medicine, Iowa City 52242."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1990.258.6.L343","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:06:16Z","timestamp":1567958776000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1990.258.6.L343"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1990,6,1]]}},"alternative-id":["10.1152\/ajplung.1990.258.6.L343"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1990.258.6.l343","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6,1]]}}}