{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T20:16:06Z","timestamp":1697487366361},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1990,6,1]]},"abstract":"<jats:p> In canine airway epithelial cells, bradykinin increases intracellular concentrations of D-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3], cytosolic calcium concentration ([Ca2+]c), and adenosine 3',5'-cyclic monophosphate (cAMP). To determine the role of these second messengers in bradykinin-stimulated Cl- secretion, we studied the secretory response to this peptide using canine tracheal monolayers mounted in Ussing chambers. Bradykinin stimulated Cl- secretion [measured as short-circuit current (Isc)] when added to submucosal or mucosal surfaces; however, secretory responses differed substantially. Submucosal addition of bradykinin induced a biphasic increase in secretion; mucosal addition induced a monophasic increase in secretion. Both responses were mediated by B2 receptors. We show that activation of bradykinin receptors can stimulate Cl- secretion in two ways. 1) Bradykinin added to either surface stimulates prostaglandin synthesis and release at the basolateral surface. This leads to activation of prostaglandin E2-sensitive receptors on the basolateral surface that are coupled to cAMP production and an increase in apical membrane Cl- conductance. 2) In addition, bradykinin added to the submucosal surface increases Ins(1,4,5)P3 and [Ca2+]c levels, which enhance basolateral K+ conductance and the electrical driving force for apical Cl- exit. Whereas secretion requires activation of apical Cl- channels, the data show that Cl- secretion can also be modulated by activation of basolateral K+ channels. These data indicate that bradykinin-induced transepithelial Cl- secretion is mediated by two independent, second messenger pathways. These results provide the first evidence for expression of both pathways in a polar fashion in an epithelial monolayer. <\/jats:p>","DOI":"10.1152\/ajplung.1990.258.6.l369","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T06:42:41Z","timestamp":1514011361000},"page":"L369-L377","source":"Crossref","is-referenced-by-count":5,"title":["Bradykinin stimulates airway epithelial Cl- secretion via two second messenger pathways"],"prefix":"10.1152","volume":"258","author":[{"given":"J. J.","family":"Smith","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Pediatrics, University of Iowa College of Medicine, Iowa City 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. D.","family":"McCann","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Pediatrics, University of Iowa College of Medicine, Iowa City 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Welsh","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Pediatrics, University of Iowa College of Medicine, Iowa City 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1990.258.6.L369","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T20:06:18Z","timestamp":1567973178000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1990.258.6.L369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1990,6,1]]}},"alternative-id":["10.1152\/ajplung.1990.258.6.L369"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1990.258.6.l369","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6,1]]}}}