{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:24:23Z","timestamp":1758637463333},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1992,8,1]]},"abstract":"<jats:p> We investigated whether loss of endothelial-derived relaxing factor (EDRF) activity in the pulmonary vessels of chronically hypoxic rats could be restored by pretreatment with L-arginine. We measured vasodilation to acetylcholine (ACh), calcium ionophore A23187, or linsidomine (Sin-1) under conditions of increased vascular tone induced by U-46619 (50 pmol\/min), as well as vasoconstriction to endothelin-1 (ET) in isolated lungs pretreated with meclofenamate (3 microM). In lungs from normoxic (N) rats, in vitro L- or D-arginine (10(-3) M) did not alter vasodilation to the endothelium-dependent agents ACh (10(-9)-10(-6) M) and A23187 (10(-9)-10(-7) M), but NG-monomethyl-L-arginine (10(-3) M) completely abolished it. In lungs from rats exposed to 3 wk of hypoxia (H), vasodilation to ACh or A23187 was fully restored after in vitro L-arginine (10(-3) M) or N alpha-benzoyl-L-arginine (5 x 10(-5) M) but remained abolished after D-arginine, L-citrulline, L-ornithine, or L-argininosuccinic acid. In vivo pretreatment of H rats with L-arginine (300 mg\/kg iv) 30 min before isolating the lung also restored vasodilation to A23187. Vasodilation to the endothelium-independent agent Sin-1 was similar in both groups of lungs and was not altered by in vitro L-arginine. L-arginine attenuated the increased pressor response to ET (300 pmol) of H rat lungs but had no effect in N rats. Our results demonstrate that loss of EDRF activity associated with hypoxic pulmonary hypertension may be reversed by supplying L-arginine. <\/jats:p>","DOI":"10.1152\/ajplung.1992.263.2.l194","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T14:36:14Z","timestamp":1514039774000},"page":"L194-L200","source":"Crossref","is-referenced-by-count":14,"title":["L-arginine restores endothelium-dependent relaxation in pulmonary circulation of chronically hypoxic rats"],"prefix":"10.1152","volume":"263","author":[{"given":"S.","family":"Eddahibi","sequence":"first","affiliation":[{"name":"Departement de Physiologie, Institut National de la Sante et de laRecherche Medicale, U 296, Hopital Henri Mondor, Creteil, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Adnot","sequence":"additional","affiliation":[{"name":"Departement de Physiologie, Institut National de la Sante et de laRecherche Medicale, U 296, Hopital Henri Mondor, Creteil, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Carville","sequence":"additional","affiliation":[{"name":"Departement de Physiologie, Institut National de la Sante et de laRecherche Medicale, U 296, Hopital Henri Mondor, Creteil, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Blouquit","sequence":"additional","affiliation":[{"name":"Departement de Physiologie, Institut National de la Sante et de laRecherche Medicale, U 296, Hopital Henri Mondor, Creteil, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Raffestin","sequence":"additional","affiliation":[{"name":"Departement de Physiologie, Institut National de la Sante et de laRecherche Medicale, U 296, Hopital Henri Mondor, Creteil, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1992.263.2.L194","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T21:01:56Z","timestamp":1567976516000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1992.263.2.L194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,8,1]]}},"alternative-id":["10.1152\/ajplung.1992.263.2.L194"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1992.263.2.l194","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,8,1]]}}}