{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:48:46Z","timestamp":1772898526522,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1994,8,1]]},"abstract":"<jats:p> The standard patch-clamp technique was employed on enzymatically digested acinar cells of submucosal glands isolated from feline trachea. ATP (-10(-3) M) evoked bidirectional current responses and an initial inward current at -80 mV (Cl- current) was followed by an outward current at 0 mV of membrane potential (K+ current). Isoproterenol (ISO) alone did not evoke any significant current responses. However, ISO augmented the ATP-induced inward and outward currents. A phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine, mimicked the augmentation by ISO. [Ca2+]i of acinar cells in isolated gland was measured using a fluorescent dye, fura 2. ATP (-10(-3) M) induced an immediate increase in [Ca2+]i followed by a prolonged plateau, and Ca2+ removal resulted in an initial increase alone without the prolonged phase. ISO also augmented the ATP-evoked increases in [Ca2+]i mainly in the plateau phases. Mucus glycoprotein (MGP) secretion was estimated by measuring trichloroacetic acid-precipitable [3H]glycoconjugates from isolated glands. ATP (-10(-3) M) evoked significant MGP secretion and ISO enhanced the ATP-induced MGP secretion. In contrast, adenosine (-10(-3) M) produced no significant responses in current, MGP secretion, or [Ca2+]i. These findings suggest that 1) P2-receptor stimulation and the resultant [Ca2+]i rise induced both electrolyte and MGP secretions and 2) ATP-induced secretion is enhanced by an adenosine 3',5'-cyclic monophosphate intracellular concentration [cAMP]i-rise after beta-receptor stimulation in airway submucosal glands. <\/jats:p>","DOI":"10.1152\/ajplung.1994.267.2.l159","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T16:35:36Z","timestamp":1514046936000},"page":"L159-L164","source":"Crossref","is-referenced-by-count":6,"title":["Extracellular ATP regulation of feline tracheal submucosal gland secretion"],"prefix":"10.1152","volume":"267","author":[{"given":"S.","family":"Shimura","sequence":"first","affiliation":[{"name":"First Department of Internal Medicine, Tohoku University School ofMedicine, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Sasaki","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Tohoku University School ofMedicine, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Nagaki","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Tohoku University School ofMedicine, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Takishima","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Tohoku University School ofMedicine, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Shirato","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Tohoku University School ofMedicine, Sendai, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1994.267.2.L159","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T21:16:24Z","timestamp":1567977384000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1994.267.2.L159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,8,1]]}},"alternative-id":["10.1152\/ajplung.1994.267.2.L159"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1994.267.2.l159","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,8,1]]}}}