{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T16:34:26Z","timestamp":1743266066611},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1995,1,1]]},"abstract":"<jats:p> The role of Ca2+ was investigated in the response of alveolar macrophages to cotton tannin, an agent implicated in the lung disease byssinosis in textile mill workers. A physiological concentration of extracellular Ca2+ was found to be required for tannin-mediated release of radiolabeled arachidonic acid (AA). Flow cytometry using indo 1 indicated that tannin caused a rapid and dose-dependent Ca2+ increase in macrophages that also required extracellular Ca2+. Ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid virtually abolished the Ca2+ influx mediated by tannin but had little effect on intracellular Ca2+ release induced by thapsigargin, N-formylmethionyl-leucylphenylalanine, or thimerosal. A mechanism for extracellular Ca2+ influx was demonstrated by rapid Mn2+ quenching of indo 1 by tannin. Verapamil inhibited tannin-mediated Ca2+ influx and AA release, but the effective concentration was 100 microM. 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid chelated all Ca2+ in the cells and effectively abolished the tannin response. Exposure to tannin was not associated with cytotoxicity, as judged by 51Cr release. The data suggest that tannin induces Ca2+ influx in alveolar macrophages, which represents an important prerequisite for a cell-signaling pathway resulting in the accumulation of free AA. <\/jats:p>","DOI":"10.1152\/ajplung.1995.268.1.l33","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T17:55:17Z","timestamp":1514051717000},"page":"L33-L40","source":"Crossref","is-referenced-by-count":2,"title":["Calcium influx is required for tannin-mediated arachidonic acid release from alveolar macrophages"],"prefix":"10.1152","volume":"268","author":[{"given":"P. J.","family":"Bates","sequence":"first","affiliation":[{"name":"Thoracic Diseases Research Unit, Mayo Clinic and Foundation, Rochester, Minnesota 55905."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. V.","family":"Ralston","sequence":"additional","affiliation":[{"name":"Thoracic Diseases Research Unit, Mayo Clinic and Foundation, Rochester, Minnesota 55905."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Vuk-Pavlovic","sequence":"additional","affiliation":[{"name":"Thoracic Diseases Research Unit, Mayo Clinic and Foundation, Rochester, Minnesota 55905."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. S.","family":"Rohrbach","sequence":"additional","affiliation":[{"name":"Thoracic Diseases Research Unit, Mayo Clinic and Foundation, Rochester, Minnesota 55905."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1995.268.1.L33","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T21:27:59Z","timestamp":1567978079000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1995.268.1.L33"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,1,1]]}},"alternative-id":["10.1152\/ajplung.1995.268.1.L33"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1995.268.1.l33","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,1,1]]}}}