{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T01:47:34Z","timestamp":1648691254348},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1995,1,1]]},"abstract":"<jats:p> Ca2+ may play an important role in mesothelial cellular responses because Ca2+ acts as an intracellular messenger in a wide variety of cellular responses in different tissues. The present study was designed to clarify the mechanisms of cytosolic Ca2+ mobilization in the mesothelial cells. Rat pleural and pericardial mesothelial cells were maintained in vitro, and the Ca2+ movement was evaluated using fura 2. Histamine (30 microM to 10 mM) induced a biphasic elevation of intracellular levels of Ca2+ concentration ([Ca2+]i) that consisted of a rapid initial transient elevation followed by a sustained elevation. Neither removal of extracellular Ca2+ nor inhibition of Ca2+ influx by 1 microM nifedipine affected the histamine-induced initial transient elevation of [Ca2+]i in mesothelial cells. Nifedipine did not block histamine-induced sustained elevation of [Ca2+]i. KCl (25 and 50 mM) elicited a biphasic elevation of [Ca2+]i. However, this KCl-induced elevation of [Ca2+]i was abolished by nifedipine treatment. Ryanodine (10 microM) induced a transient elevation of [Ca2+]i in Ca(2+)-free solution. The histamine-induced elevation of [Ca2+]i was completely blocked by H1-receptor antagonists. These results suggest that the mesothelial cells have three pathways to increase [Ca2+]i: release from intracellular Ca2+ stores, Ca2+ influx through L-type voltage-dependent Ca2+ channels, and receptor-operated Ca2+ channels. <\/jats:p>","DOI":"10.1152\/ajplung.1995.268.1.l63","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T12:55:17Z","timestamp":1514033717000},"page":"L63-L70","source":"Crossref","is-referenced-by-count":2,"title":["Role of intra- and extracellular calcium stores in mesothelial cell response to histamine"],"prefix":"10.1152","volume":"268","author":[{"given":"K.","family":"Ito","sequence":"first","affiliation":[{"name":"Department of Comparative Pathophysiology, Faculty of Agriculture, University of Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kuwahara","sequence":"additional","affiliation":[{"name":"Department of Comparative Pathophysiology, Faculty of Agriculture, University of Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Sugano","sequence":"additional","affiliation":[{"name":"Department of Comparative Pathophysiology, Faculty of Agriculture, University of Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kuwahara","sequence":"additional","affiliation":[{"name":"Department of Comparative Pathophysiology, Faculty of Agriculture, University of Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1995.268.1.L63","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T17:28:01Z","timestamp":1567963681000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1995.268.1.L63"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,1,1]]}},"alternative-id":["10.1152\/ajplung.1995.268.1.L63"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1995.268.1.l63","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,1,1]]}}}