{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T19:51:23Z","timestamp":1771530683830,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1995,3,1]]},"abstract":"<jats:p> The mouse surfactant protein B (SP-B) gene was isolated from a DBA\/2J EMBL3 genomic library, and the nucleotide sequence and transcriptional start site was determined. The SP-B gene was comprised of 11 exons and 10 introns and spanned 9,882 bases. Repetitive elements of the B1, B2, and R families and several other motifs were located within nontranslated regions of the gene. The 5' flanking region of the gene was utilized to express a bacterial chloramphenicol acetyl transferase reporter gene in mouse and human pulmonary adenocarcinoma cell lines. Transcriptionally active regions of the promoter were identified that determined lung epithelial cell-specific gene expression. Regions of the gene promoter shared between the mouse and human SP-B gene were used to locate putative transcription factor binding sites for thyroid transcription factor-1 and hepatocyte nuclear factor-3. The 5' flanking region of the murine SP-B contains cell-specific cis-active elements that regulate SP-B expression in the respiratory epithelium. <\/jats:p>","DOI":"10.1152\/ajplung.1995.268.3.l381","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T17:59:15Z","timestamp":1514051955000},"page":"L381-L389","source":"Crossref","is-referenced-by-count":7,"title":["Structure and function of the mouse surfactant protein B gene"],"prefix":"10.1152","volume":"268","author":[{"given":"M. A.","family":"Bruno","sequence":"first","affiliation":[{"name":"Children's Hospital Research Foundation, Division of PulmonaryBiology, Children's Hospital Medical Center, Cincinnati, Ohio 45229."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Bohinski","sequence":"additional","affiliation":[{"name":"Children's Hospital Research Foundation, Division of PulmonaryBiology, Children's Hospital Medical Center, Cincinnati, Ohio 45229."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"Carter","sequence":"additional","affiliation":[{"name":"Children's Hospital Research Foundation, Division of PulmonaryBiology, Children's Hospital Medical Center, Cincinnati, Ohio 45229."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. A.","family":"Foss","sequence":"additional","affiliation":[{"name":"Children's Hospital Research Foundation, Division of PulmonaryBiology, Children's Hospital Medical Center, Cincinnati, Ohio 45229."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"Whitsett","sequence":"additional","affiliation":[{"name":"Children's Hospital Research Foundation, Division of PulmonaryBiology, Children's Hospital Medical Center, Cincinnati, Ohio 45229."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1995.268.3.L381","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T21:26:34Z","timestamp":1567977994000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1995.268.3.L381"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,3,1]]}},"alternative-id":["10.1152\/ajplung.1995.268.3.L381"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1995.268.3.l381","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3,1]]}}}