{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T13:16:48Z","timestamp":1648991808817},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1996,8,1]]},"abstract":"<jats:p> This study examined the effect of inhaled nitric oxide (NO) on lung neutrophil accumulation and endothelial-dependent and -independent guanosine 3',5'-cyclic monophosphate (cGMP)-mediated mechanisms of pulmonary vasorelaxation after mesenteric ischemia-reperfusion (I\/R) in mechanically ventilated rats. Inhaled NO (20 ppm) was administered in two protocols: 1) throughout mesenteric I\/R and 2) during mesenteric reperfusion alone. Concentration-response curves were generated (10(-9) to 10(-8) M) for acetylcho-line (ACh), A23187, and sodium nitroprusside (SNP) in isolated pulmonary arterial rings preconstricted with phenylephrine. Lung neutrophil accumulation [myeloperoxidase assay (MPO)] was significantly increased from 2.4 +\/- 0.2 units\/g lung wt in controls to 10.3 +\/- 0.4 after 1 h of superior mesenteric artery occlusion and 2 h of reperfusion. Lung MPO activity was not different from controls in rats receiving inhaled NO either 1) during mesenteric I\/R or during mesenteric reperfusion alone. The concentration-response curves demonstrated significant impairment of pulmonary vasorelaxation by endothelial-dependent mechanisms (response to ACh and A23187) but not endothelial-independent pulmonary vasorelaxation (response to SNP) after mesenteric I\/R. This pulmonary vasomotor dysfunction was prevented by administration of inhaled NO during either mesenteric I\/R or during mesenteric reperfusion alone. We conclude that inhaled NO prevents lung neutrophil accumulation and pulmonary vascular endothelial dysfunction after mesenteric I\/R. <\/jats:p>","DOI":"10.1152\/ajplung.1996.271.2.l326","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T08:12:32Z","timestamp":1514016752000},"page":"L326-L331","source":"Crossref","is-referenced-by-count":3,"title":["Inhaled nitric oxide prevents pulmonary endothelial dysfunction after mesenteric ischemia-reperfusion"],"prefix":"10.1152","volume":"271","author":[{"given":"D. A.","family":"Fullerton","sequence":"first","affiliation":[{"name":"University of Colorado Health Sciences Center, Denver 80262,USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. H.","family":"Eisenach","sequence":"additional","affiliation":[{"name":"University of Colorado Health Sciences Center, Denver 80262,USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"R. C.","family":"McIntyre","sequence":"additional","affiliation":[{"name":"University of Colorado Health Sciences Center, Denver 80262,USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. S.","family":"Friese","sequence":"additional","affiliation":[{"name":"University of Colorado Health Sciences Center, Denver 80262,USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. C.","family":"Sheridan","sequence":"additional","affiliation":[{"name":"University of Colorado Health Sciences Center, Denver 80262,USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. B.","family":"Roe","sequence":"additional","affiliation":[{"name":"University of Colorado Health Sciences Center, Denver 80262,USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Agrafojo","sequence":"additional","affiliation":[{"name":"University of Colorado Health Sciences Center, Denver 80262,USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Banerjee","sequence":"additional","affiliation":[{"name":"University of Colorado Health Sciences Center, Denver 80262,USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. H.","family":"Harken","sequence":"additional","affiliation":[{"name":"University of Colorado Health Sciences Center, Denver 80262,USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1996.271.2.L326","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:47:42Z","timestamp":1567961262000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1996.271.2.L326"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,8,1]]}},"alternative-id":["10.1152\/ajplung.1996.271.2.L326"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1996.271.2.l326","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,8,1]]}}}