{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:39:47Z","timestamp":1790768387604,"version":"4.1.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1997,2,1]]},"abstract":"<jats:p> We found that surfactant protein A (SP-A) enhances phagocytosis of Klebsiella pneumoniae K21a but not of K2 serotypes by alveolar macrophages. SP-A interacted with the capsule of K21a (containing Man alpha1 Man sequences) as shown by SP-A-induced agglutination of the bacteria, by binding of SP-A-coated particles onto the bacterial surface, and by binding of SP-A to immobilized parent K21a strain and recombinant strains that switched their capsule from K2 to K21a. In contrast, only marginal binding of SP-A to K2 parent strain (lacking this sequence) could be detected. Furthermore, binding of capsular polysaccharide of K21a to immobilized SP-A was inhibited by mannan but not by lipopolysaccharide and K2 capsular polysaccharide. SP-A-treated macrophages bound increased numbers of parent K21a strain and recombinant strains of K21a capsule type but considerably less parent K2 strain. SP-A also enhanced killing of K21a strains by macrophages. The enhanced binding of K21a by macrophages pretreated with SP-A was inhibited by mannan, suggesting that binding is mediated by the mannose receptor on macrophages. We conclude that SP-A increases phagocytosis of the Klebsiella by two mechanisms, one of which is by serving as an opsonin, which binds to the capsular polysaccharides of the bacteria and potentially to SP-A receptors on the macrophages, and the other by activating the macrophages, resulting in increased activity of the mannose receptor. <\/jats:p>","DOI":"10.1152\/ajplung.1997.272.2.l344","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T12:28:33Z","timestamp":1514032113000},"page":"L344-L352","source":"Crossref","is-referenced-by-count":30,"title":["SP-A enhances phagocytosis of Klebsiella by interaction with capsular polysaccharides and alveolar macrophages"],"prefix":"10.1152","volume":"272","author":[{"given":"K.","family":"Kabha","sequence":"first","affiliation":[{"name":"Department of Human Microbiology, Sackler Faculty of Medicine,Tel-Aviv University, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Schmegner","sequence":"additional","affiliation":[{"name":"Department of Human Microbiology, Sackler Faculty of Medicine,Tel-Aviv University, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Keisari","sequence":"additional","affiliation":[{"name":"Department of Human Microbiology, Sackler Faculty of Medicine,Tel-Aviv University, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Parolis","sequence":"additional","affiliation":[{"name":"Department of Human Microbiology, Sackler Faculty of Medicine,Tel-Aviv University, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Schlepper-Schaeffer","sequence":"additional","affiliation":[{"name":"Department of Human Microbiology, Sackler Faculty of Medicine,Tel-Aviv University, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Ofek","sequence":"additional","affiliation":[{"name":"Department of Human Microbiology, Sackler Faculty of Medicine,Tel-Aviv University, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1997.272.2.L344","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T20:43:55Z","timestamp":1567975435000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1997.272.2.L344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,2,1]]}},"alternative-id":["10.1152\/ajplung.1997.272.2.L344"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1997.272.2.l344","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,2,1]]}}}