{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T03:37:07Z","timestamp":1648697827382},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1997,7,1]]},"abstract":"<jats:p> We studied the mechanism of degranulation caused by Ca(2+)-activated K+ channels (KCa channels) in eosinophils isolated from mildly atopic donors using negative immunoselection. Stimulation of eosinophils with 0.1 microM platelet-activating factor (PAF) caused activation of single channels as recorded by the cell-attached patch-clamp technique. These channels were selectively permeable to K+ because the reversal potential was close to the equilibrium potential for K+. However, the channels were not permeable to Na+ or Cl- as demonstrated by ion substitution experiments. The calcium ionophore A-23187, at 1 microM, increased the K+ channel activity in the presence of Ca2+ in the external perfusate but did not induce channel activity in the absence of Ca2+. Similar results were obtained with another calcium ionophore, ionomycin (1 microM), and the Ca(2+)-releasing agent thapsigargin (10 microM). K+ channels activated by PAF and A-23187 had similar characteristics: two levels of single-channel conductances were observed, 10 +\/- 1.5 and 22 +\/- 1.7 pS as induced by PAF and 11 +\/- 1.3 and 24 +\/- 1.9 pS by A-23187; the mean open times of the large-conductance channels were 1.45 +\/- 0.3 ms as induced by PAF and 1.26 +\/- 0.5 ms by A-23187. These results indicate that PAF activates KCa channels. Both KCa currents and major basic protein release caused by A-23187 were blocked by quinidine. It is suggested that KCa channels are associated with granule secretion in human eosinophils. <\/jats:p>","DOI":"10.1152\/ajplung.1997.273.1.l16","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T08:23:46Z","timestamp":1514017426000},"page":"L16-L21","source":"Crossref","is-referenced-by-count":0,"title":["Association of granular exocytosis with Ca(2+)-activated K+ channels in human eosinophils"],"prefix":"10.1152","volume":"273","author":[{"given":"M.","family":"Saito","sequence":"first","affiliation":[{"name":"Department of Molecular Pharmacology and Biological Chemistry,Northwestern University Medical School, Chicago 60611-3008, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Biological Chemistry,Northwestern University Medical School, Chicago 60611-3008, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. M.","family":"Munoz","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Biological Chemistry,Northwestern University Medical School, Chicago 60611-3008, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Herrnreiter","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Biological Chemistry,Northwestern University Medical School, Chicago 60611-3008, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Oyaizu","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Biological Chemistry,Northwestern University Medical School, Chicago 60611-3008, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kasugai","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Biological Chemistry,Northwestern University Medical School, Chicago 60611-3008, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Narahashi","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Biological Chemistry,Northwestern University Medical School, Chicago 60611-3008, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. R.","family":"Leff","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Biological Chemistry,Northwestern University Medical School, Chicago 60611-3008, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1997.273.1.L16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T16:50:48Z","timestamp":1567961448000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1997.273.1.L16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,7,1]]}},"alternative-id":["10.1152\/ajplung.1997.273.1.L16"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1997.273.1.l16","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,7,1]]}}}