{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T11:29:49Z","timestamp":1658834989274},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1997,7,1]]},"abstract":"<jats:p> This study tested the hypothesis that airway relaxation to furosemide is mediated via the Na-K-2Cl cotransporter. If this mechanism exists in airway smooth muscle like in vascular smooth muscle, changes in airway relaxation should be associated with changes in Na-K-2Cl cotransporter function, and both should be substrate dependent. Tracheal rings from newborn guinea pigs were bathed in standard (STD) or varying low Cl- concentration ([Cl-]) N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES). Isometric relaxation to 300 microM furosemide or 10(-8) to 10(-5) M salbutamol was measured. Airway segments were incubated with rubidium-86 (86Rb) in STD or varying low [Cl-] HEPES, with and without 300 microM furosemide or 25 microM salbutamol. Furosemide was unable to reduce 86Rb uptake at 10 mM [Cl-], although relaxation was still observed in 10 mM [Cl-]. Salbutamol did not affect 86Rb uptake. This study demonstrated that there is a furosemide-sensitive Na-K-2Cl cotransporter in newborn guinea pig trachea. However, the effect of furosemide on cotransporter function did not always directly correspond to differences in relaxation, suggesting that the Na-K-2Cl cotransporter may play a major, but not exclusive, role in furosemide-induced airway relaxation. <\/jats:p>","DOI":"10.1152\/ajplung.1997.273.1.l211","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T13:23:46Z","timestamp":1514035426000},"page":"L211-L216","source":"Crossref","is-referenced-by-count":6,"title":["Furosemide-induced airway relaxation in guinea pigs: relation to Na-K-2Cl cotransporter function"],"prefix":"10.1152","volume":"273","author":[{"given":"S. L.","family":"Lavallee","sequence":"first","affiliation":[{"name":"Department of Clinical Investigation, Tripler Army Medical Center,Honolulu, Hawaii 96859, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. M.","family":"Iwamoto","sequence":"additional","affiliation":[{"name":"Department of Clinical Investigation, Tripler Army Medical Center,Honolulu, Hawaii 96859, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Claybaugh","sequence":"additional","affiliation":[{"name":"Department of Clinical Investigation, Tripler Army Medical Center,Honolulu, Hawaii 96859, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. V.","family":"Dressel","sequence":"additional","affiliation":[{"name":"Department of Clinical Investigation, Tripler Army Medical Center,Honolulu, Hawaii 96859, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. K.","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Clinical Investigation, Tripler Army Medical Center,Honolulu, Hawaii 96859, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. T.","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Clinical Investigation, Tripler Army Medical Center,Honolulu, Hawaii 96859, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.1997.273.1.L211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T20:51:02Z","timestamp":1567975862000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.1997.273.1.L211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,7,1]]}},"alternative-id":["10.1152\/ajplung.1997.273.1.L211"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.1997.273.1.l211","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,7,1]]}}}