{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T06:23:51Z","timestamp":1791699831644,"version":"4.3.4"},"reference-count":34,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[1998,4,1]]},"abstract":"<jats:p>Neonates with congenital diaphragmatic hernia (DH) die of pulmonary hypoplasia and persistent pulmonary hypertension. We used immunohistochemical localization of \u03b1-smooth muscle actin (\u03b1-SMA), platelet endothelial cell adhesion molecule (PECAM)-1, thyroid transcription factor (TTF)-1, surfactant protein (SP) A, SP-C, and competitive RT-PCR quantitation of TTF-1, SP-A, SP-C, and \u03b1-SMA mRNA expression to characterize the epithelial and vascular phenotype of lungs from ICR fetal mice with a nitrofen-induced DH. Nitrofen (25 mg) was gavage fed to pregnant mice on day 8 of gestation. Fetal mice were delivered on day 17. The diaphragm was examined for a defect, and the lungs were either fixed, sectioned, and immunostained or processed for mRNA isolation. In comparison with control lungs, DH lungs showed increased expression of \u03b1-SMA mRNA, fewer and more muscular arterioles (\u03b1-SMA), less well-developed capillary networks (PECAM-1), delayed epithelial development marked by a persistence of TTF-1 in the periphery, and decreased SP-A mRNA and SP-A expression. These data suggest that in the murine nitrofen-induced DH, as in human congenital DH, pulmonary insufficiency is due to an inhibition of peripheral pulmonary development including terminal airway and vascular morphogenesis.<\/jats:p>","DOI":"10.1152\/ajplung.1998.274.4.l636","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T14:06:58Z","timestamp":1514038018000},"page":"L636-L646","source":"Crossref","is-referenced-by-count":17,"title":["Inhibition of vascular and epithelial differentiation in murine nitrofen-induced diaphragmatic hernia"],"prefix":"10.1152","volume":"274","author":[{"given":"C.","family":"Coleman","sequence":"first","affiliation":[{"name":"Division of Pediatric Surgery and Developmental Biology Program, Childrens Hospital Los Angeles Research Institute, Los Angeles 90027; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for Craniofacial and Molecular Biology, University of Southern California Schools of Medicine and Dentistry, Los Angeles, California 90033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Gupta","sequence":"additional","affiliation":[{"name":"Division of Pediatric Surgery and Developmental Biology Program, Childrens Hospital Los Angeles Research Institute, Los Angeles 90027; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Buckley","sequence":"additional","affiliation":[{"name":"Division of Pediatric Surgery and Developmental Biology Program, Childrens Hospital Los Angeles Research Institute, Los Angeles 90027; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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