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Using a pH-sensitive fluorescent probe, 2\u2032,7\u2032-bis(2-carboxyethyl)-5(6)-carboxyfluorescein, to measure changes in intracellular pH (pH<jats:sub>i<\/jats:sub>), we found that 1) EGF and 12- O-tetradecanoylphorbol 13-acetate (TPA; a phorbol ester) both stimulate the ethylisopropyl amiloride-sensitive Na<jats:sup>+<\/jats:sup>-H<jats:sup>+<\/jats:sup>exchanger; 2) TPA-induced alkalosis can be blocked by protein kinase C (PKC) inhibitors (chelerythrine and staurosporine) or by PKC downregulation, indicating that PKC activation is involved in the stimulation of the Na<jats:sup>+<\/jats:sup>-H<jats:sup>+<\/jats:sup>exchanger. However, TPA-induced alkalosis is not blocked by tyrosine kinase inhibitors; and 3) the stimulatory effect of EGF on the Na<jats:sup>+<\/jats:sup>-H<jats:sup>+<\/jats:sup>exchanger acts via stimulation of tyrosine kinase-receptor activity because it is inhibited by tyrosine kinase inhibitors (genistein, lavendustin A, and herbimycin A). It also involves PKC activation because EGF-induced alkalosis was blocked by PKC inhibitors. These results suggest that PKC activation is one of the downstream signals for EGF-induced activation of the Na<jats:sup>+<\/jats:sup>-H<jats:sup>+<\/jats:sup>exchanger in primary cultures of human pleural mesothelial cells.<\/jats:p>","DOI":"10.1152\/ajplung.1998.274.5.l665","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T22:30:50Z","timestamp":1514068250000},"page":"L665-L672","source":"Crossref","is-referenced-by-count":10,"title":["PKC activation is required by EGF-stimulated Na<sup>+<\/sup>-H<sup>+<\/sup>exchanger in human pleural mesothelial cells"],"prefix":"10.1152","volume":"274","author":[{"given":"Yuang-Shuang","family":"Liaw","sequence":"first","affiliation":[{"name":"Laboratory of Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan-Chyr","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory of Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong-Jen","family":"Yu","sequence":"additional","affiliation":[{"name":"Laboratory of Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sow-Hsong","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwen-Tay","family":"Luh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuh-Jeng","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Physiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Lin","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Physiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"5592","DOI":"10.1016\/S0021-9258(18)45614-1","volume":"262","author":"Akiyama T.","year":"1987","journal-title":"J. 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