{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:23:26Z","timestamp":1759332206375},"reference-count":28,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2000,1,1]]},"abstract":"<jats:p>The cellular mechanisms by which pulmonary surfactant exerts its effects, including anti-inflammatory or proinflammatory effects, have remained elusive. To address the issue of whether plasma membrane modifications represent a target for these mechanisms, we designed an experimental protocol involving the determination of changes in cAMP levels under membrane-dependent or -independent stimulatory pathways. The effects of a modified natural porcine surfactant, Curosurf, and the major surfactant protein A were evaluated on resting and stimulated cAMP levels of human monocytes. We found that agents that elevate intracellular cAMP exhibit different susceptibilities toward a preexposure to Curosurf. The rise in cAMP induced by membrane-active agents such as cholera toxin or the diterpene forskolin was significantly inhibited by monocyte preexposure to Curosurf. In contrast, the rise in cAMP induced by the membrane-permeant phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine or by the Bordetella pertussis toxin adenylate cyclase-hemolysin was unaffected by Curosurf. Surfactant protein A did not affect either cAMP levels or the inhibitory capacity of Curosurf. We suggest that a plasma membrane-associated event affecting the mechanism underlying the effects of cholera toxin or forskolin is involved in the inhibition of cAMP accumulation caused by Curosurf.<\/jats:p>","DOI":"10.1152\/ajplung.2000.278.1.l99","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T00:43:09Z","timestamp":1514076189000},"page":"L99-L104","source":"Crossref","is-referenced-by-count":11,"title":["Curosurf modulates cAMP accumulation in human monocytes through a membrane-controlled mechanism"],"prefix":"10.1152","volume":"278","author":[{"given":"Fran\u00e7oise","family":"Pinot","sequence":"first","affiliation":[{"name":"Laboratoire de Physiologie Respiratoire, Unit\u00e9 de Formation et de Recherche Cochin Port-Royal, Assistance Publique-H\u00f4pitaux de Paris Universit\u00e9 Paris V, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herv\u00e9","family":"Walti","sequence":"additional","affiliation":[{"name":"Service de M\u00e9decine N\u00e9onatale, H\u00f4pital Cochin Port-Royal, 75014 Paris, France; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk P.","family":"Haagsman","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Biochemistry and Graduate School of Animal Health, 3508 TD Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara S.","family":"Polla","sequence":"additional","affiliation":[{"name":"Laboratoire de Physiologie Respiratoire, Unit\u00e9 de Formation et de Recherche Cochin Port-Royal, Assistance Publique-H\u00f4pitaux de Paris Universit\u00e9 Paris V, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Bachelet","sequence":"additional","affiliation":[{"name":"Laboratoire de Physiologie Respiratoire, Unit\u00e9 de Formation et de Recherche Cochin Port-Royal, Assistance Publique-H\u00f4pitaux de Paris Universit\u00e9 Paris V, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.14.5.8624255"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(88)90044-1"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1379\/1466-1268(1998)003<0168:FCIARA>2.3.CO;2"},{"key":"B4","first-page":"47","volume":"84","author":"Batenburg J.","year":"1995","journal-title":"Surfactant Therapy for Lung Disease"},{"key":"B5","first-page":"1","volume":"22","author":"Beavo J. A.","year":"1988","journal-title":"Adv. Second Messengers Phosphoprotein Res."},{"key":"B6","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.1128\/IAI.61.9.3583-3589.1993","volume":"61","author":"Betsou F.","year":"1993","journal-title":"Infect. Immun."},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.6.2669"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(93)90321-6"},{"key":"B9","doi-asserted-by":"crossref","first-page":"21470","DOI":"10.1016\/S0021-9258(20)88206-4","volume":"264","author":"Gierschik P.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.67.1.305"},{"key":"B11","doi-asserted-by":"crossref","first-page":"13877","DOI":"10.1016\/S0021-9258(18)47873-8","volume":"262","author":"Haagsman H. P.","year":"1987","journal-title":"J. Biol. Chem."},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/9.5.520"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1997.273.2.L382"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1997.272.6.L1070"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1997.272.5.L996"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.17.9695"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(98)00542-6"},{"key":"B18","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.1016\/S0021-9258(19)77178-6","volume":"245","author":"Reinmann E. M.","year":"1971","journal-title":"J. Biol. Chem."},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1159\/000417829"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.6.3363"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199107000-00015"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.10.4.8136155"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1996.270.4.L677"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/2.1.91"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199701000-00018"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1994.267.6.L660"},{"key":"B27","first-page":"410","volume":"71","author":"Wiernik A.","year":"1987","journal-title":"Eur. J. Respir. Dis."},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1997.77.4.931"}],"container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.2000.278.1.L99","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T04:18:55Z","timestamp":1660191535000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.2000.278.1.L99"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,1,1]]}},"alternative-id":["10.1152\/ajplung.2000.278.1.L99"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.2000.278.1.l99","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,1,1]]}}}