{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:10:10Z","timestamp":1751173810308,"version":"3.41.0"},"reference-count":31,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2000,5,1]]},"abstract":"<jats:p>Nitric oxide (NO) relaxes vascular smooth muscle in part through an accumulation of cGMP in the target cells. We hypothesized that a similar effect may also exist on collagen gel contraction mediated by human fetal lung (HFL1) fibroblasts, a model of wound contraction. To evaluate this, HFL1 cells were cultured in three-dimensional type I collagen gels and floated in serum-free DMEM with and without various NO donors. Gel size was measured with an image analyzer. Sodium nitroprusside (SNP, 100 \u03bcM) significantly augmented collagen gel contraction by HFL1 cells (78.5 \u00b1 0.8 vs. 58.3 \u00b1 2.1, P &lt; 0.01), whereas S-nitroso- N-acetylpenicillamine, 5-amino-3-(4-morpholinyl)-1,2,3-oxadiazolium chloride, NONOate, and N<jats:sup>G<\/jats:sup>-monomethyl-l-arginine did not affect the contraction. Sodium ferricyanide, sodium nitrate, or sodium nitrite was not active. The augmentory effect of SNP could not be blocked by 1 H-[1,2,4]-oxadiazolo-[4,3- a]-quinoxalin-1-one, whereas it was partially reversed by 8-(4-chlorophenylthio) (CPT)-cGMP. To further explore the mechanisms by which SNP acted, fibronectin and PGE<jats:sub>2<\/jats:sub>production were measured by immunoassay after 2 days of gel contraction. SNP inhibited PGE<jats:sub>2<\/jats:sub>production and increased fibronectin production by HFL1 cells in a concentration-dependent manner. CPT-cGMP had opposite effects on fibronectin and PGE<jats:sub>2<\/jats:sub>production. Addition of exogenous PGE<jats:sub>2<\/jats:sub>blocked SNP-augmented contraction and fibronectin production by HFL1 cells. Therefore, SNP was able to augment human lung fibroblast-mediated collagen gel contraction, an effect that appears to be independent of NO production and not mediated through cGMP. Decreased PGE<jats:sub>2<\/jats:sub>production and augmented fibronectin production may have a role in this effect. These data suggest that human lung fibroblasts in three-dimensional type I collagen gels respond distinctly to SNP by mechanisms unrelated to the NO-cGMP pathway.<\/jats:p>","DOI":"10.1152\/ajplung.2000.278.5.l1032","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T00:34:27Z","timestamp":1514075667000},"page":"L1032-L1038","source":"Crossref","is-referenced-by-count":4,"title":["Sodium nitroprusside augments human lung fibroblast collagen gel contraction independently of NO-cGMP pathway"],"prefix":"10.1152","volume":"278","author":[{"given":"X. D.","family":"Liu","sequence":"first","affiliation":[{"name":"Pulmonary and Critical Care Medicine Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198-5300"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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