{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:46:41Z","timestamp":1773301601611,"version":"3.50.1"},"reference-count":39,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2000,8,1]]},"abstract":"<jats:p>The present study was undertaken to determine whether rat carotid bodies express adenosine (Ado) A<jats:sub>2A<\/jats:sub>receptors and whether this receptor is involved in the cellular response to hypoxia. Our results demonstrate that rat carotid bodies express the A<jats:sub>2A<\/jats:sub>and A<jats:sub>2B<\/jats:sub>Ado receptor mRNAs but not the A<jats:sub>1<\/jats:sub>or A<jats:sub>3<\/jats:sub>receptor mRNAs as determined by reverse transcriptase-polymerase chain reaction. In situ hybridization confirmed the expression of the A<jats:sub>2A<\/jats:sub>receptor mRNA. Immunohistochemical studies further showed that the A<jats:sub>2A<\/jats:sub>receptor is expressed in the carotid body and that it is colocalized with tyrosine hydroxylase in type I cells. Whole cell voltage-clamp studies using isolated type I cells showed that Ado inhibited the voltage-dependent Ca<jats:sup>2+<\/jats:sup>currents and that this inhibition was abolished by the selective A<jats:sub>2A<\/jats:sub>receptor antagonist ZM-241385. Ca<jats:sup>2+<\/jats:sup>imaging studies using fura 2 revealed that exposure to severe hypoxia induced elevation of intracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) in type I cells and that extracellularly applied Ado significantly attenuated the hypoxia-induced elevation of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. Taken together, our findings indicate that A<jats:sub>2A<\/jats:sub>receptors are present in type I cells and that activation of A<jats:sub>2A<\/jats:sub>receptors modulates Ca<jats:sup>2+<\/jats:sup>accumulation during hypoxia. This mechanism may play a role in regulating intracellular Ca<jats:sup>2+<\/jats:sup>homeostasis and cellular excitability during hypoxia.<\/jats:p>","DOI":"10.1152\/ajplung.2000.279.2.l273","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T19:18:23Z","timestamp":1514056703000},"page":"L273-L282","source":"Crossref","is-referenced-by-count":45,"title":["Gene expression and function of adenosine A<sub>2A<\/sub>receptor in the rat carotid body"],"prefix":"10.1152","volume":"279","author":[{"given":"Shuichi","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Physiology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Conforti","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension, Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio 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