{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T16:46:53Z","timestamp":1776530813895,"version":"3.51.2"},"reference-count":28,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:p> Pulmonary interstitial pressure was measured via micropuncture in anesthetized rabbits in normoxia and after breathing 12% O<jats:sub>2<\/jats:sub>. In normoxia [arterial Po <jats:sub>2<\/jats:sub> = 88 \u00b1 2 (SD) mmHg], pulmonary arterial pressure and pulmonary interstitial pressure were 16 \u00b1 8 and \u22129.6 \u00b1 2 cmH<jats:sub>2<\/jats:sub>O, respectively. After 6 h of hypoxia (arterial Po <jats:sub>2<\/jats:sub> = 39 \u00b1 16 mmHg), the corresponding values were 30 \u00b1 8 and 3.5 \u00b1 2.5 cmH<jats:sub>2<\/jats:sub>O ( P &lt; 0.05). Pulmonary interstitial proteoglycan extractability, evaluated by hexuronate assay after 0.4 M guanidinium hydrochloride extraction, was 12.3, 32.4, and 60.6 \u03bcg\/g wet tissue in normoxia and after 3 and 6 h of hypoxia, respectively, indicating a weakening of the noncovalent bonds linking proteoglycans to other extracellular matrix components. Gel filtration chromatography showed an increased fragmentation of chondroitin sulfate- and heparan sulfate-proteoglycans during hypoxic exposure, accounting for a loss of extracellular matrix native architecture and basement membrane structure. Gelatin zymography demonstrated increased amounts of the proteolytically activated form of gelatinase B (matrix metalloproteinase-9) after hypoxic exposure, providing evidence that the activation of proteinases may play a role in hypoxia-induced lung injury. <\/jats:p>","DOI":"10.1152\/ajplung.2001.280.5.l881","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T14:53:48Z","timestamp":1514040828000},"page":"L881-L887","source":"Crossref","is-referenced-by-count":65,"title":["Pulmonary interstitial pressure and tissue matrix structure in acute hypoxia"],"prefix":"10.1152","volume":"280","author":[{"given":"Giuseppe","family":"Miserocchi","sequence":"first","affiliation":[{"name":"Department of Experimental and Environmental Medicine and Biotechnology, University of Milano-Bicocca, 20052 Monza;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Passi","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Biomedical Sciences, University of Insubria, 21100 Varese, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Negrini","sequence":"additional","affiliation":[{"name":"Department of Medicine, Surgery and Dentistry, University of Milano, 20142 Milan; 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