{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T20:44:16Z","timestamp":1774125856193,"version":"3.50.1"},"reference-count":26,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2001,10,1]]},"abstract":"<jats:p> Ion transport and the electric profile of distal airways of sheep lungs were studied in a miniature polypropylene chamber with a 1-mm aperture. Small airways with an inner diameter &lt; 1 mm were isolated, opened longitudinally, and then mounted as a flat sheet onto the 1-mm aperture where it was glued and secured with an O-ring. Both sides of the tissue were bathed with identical physiological solutions at 37\u00b0C and oxygenated. Pooled data from 27 distal airways showed an inner airway diameter of 854 \u00b1 22 (SE) \u03bcm and a transepithelial potential difference (PD) of 1.86 \u00b1 0.29 mV, lumen negative. Short-circuit current ( I <jats:sub>sc<\/jats:sub>) was 25 \u00b1 3.5 \u03bcA\/cm<jats:sup>2<\/jats:sup>, tissue resistance was 96 \u00b1 14 \u03a9, and conductance was 15.2 \u00b1 1.7 mS\/cm<jats:sup>2<\/jats:sup>. At baseline, amiloride-sensitive Na transport accounted for 51% of I <jats:sub>sc<\/jats:sub> (change in I <jats:sub>sc<\/jats:sub> = 9.7 \u00b1 2.6 \u03bcA\/cm<jats:sup>2<\/jats:sup>; n = 8 airways), corresponding to 0.36 \u03bceq \u00b7 cm<jats:sup>\u22122<\/jats:sup> \u00b7 h<jats:sup>\u22121<\/jats:sup>. Treatment with 0.1 mM bumetanide did not reduce the I <jats:sub>sc<\/jats:sub>( n = 5 airways). Exposure to 1 \u03bcM Ca ionophore A-23187 raised the I <jats:sub>sc<\/jats:sub> by 9 \u03bcA\/cm<jats:sup>2<\/jats:sup>(47%; P &lt; 0.03; n = 6 airways). The latter effect was blunted by bumetanide. Carbachol at 1 \u03bcM provoked a biphasic response, an initial rapid rise in I <jats:sub>sc<\/jats:sub>followed by a decline ( n = 3 airways). There was no significant increase in PD or I <jats:sub>sc<\/jats:sub> in response to isoproterenol or dibutyryl cAMP. The data suggest that Na absorption constitutes at least 50% of baseline transport activity. Cl or other anion secretion such as HCO<jats:sub>3<\/jats:sub> appears to be present and could be stimulated by raising intracellular Ca. <\/jats:p>","DOI":"10.1152\/ajplung.2001.281.4.l1028","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T15:51:42Z","timestamp":1514044302000},"page":"L1028-L1034","source":"Crossref","is-referenced-by-count":12,"title":["Ion transport by sheep distal airways in a miniature chamber"],"prefix":"10.1152","volume":"281","author":[{"given":"Faiq J.","family":"Al-Bazzaz","sequence":"first","affiliation":[{"name":"Respiratory and Critical Care Section, Veterans Affairs Chicago Healthcare System-Westside Division, and Department of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Gailey","sequence":"additional","affiliation":[{"name":"Respiratory and Critical Care Section, Veterans Affairs Chicago Healthcare System-Westside Division, and Department of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp017973"},{"key":"B2","doi-asserted-by":"crossref","first-page":"125S","DOI":"10.1183\/09031936.96.09010125","volume":"9","author":"Al-Bazzaz F","year":"1996","journal-title":"Eur Respir J"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3109\/01902148109052308"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1994.267.2.L193"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1991.260.6.L594"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1992.73.5.2021"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1994.267.1.L79"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1007\/BF00216752"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1980.48.1.169"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1990.258.2.L25"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197806082982303"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1990.258.2.L75"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.258.2.C318"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112311"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1136\/thx.23.6.670"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019240"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1126\/science.173.3992.146"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)80519-2"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1996.270.2.L289"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1007\/BF00584830"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092050304"},{"key":"B23","doi-asserted-by":"crossref","first-page":"1546","DOI":"10.1152\/ajplegacy.1976.231.5.1546","volume":"231","author":"Marin MG","year":"1976","journal-title":"Am J Physiol"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016122"},{"key":"B27","first-page":"24","volume":"3","author":"Steel DM","year":"1994","journal-title":"Epithelial Cell Biol"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/141.3.584"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1987.67.4.1143"}],"container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.2001.281.4.L1028","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T17:45:24Z","timestamp":1567964724000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.2001.281.4.L1028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10,1]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,10,1]]}},"alternative-id":["10.1152\/ajplung.2001.281.4.L1028"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.2001.281.4.l1028","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10,1]]}}}