{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T09:57:48Z","timestamp":1772272668064,"version":"3.50.1"},"reference-count":28,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2001,10,1]]},"abstract":"<jats:p>We examined the regulatory role of cytosolic phospholipase A<jats:sub>2<\/jats:sub>(cPLA<jats:sub>2<\/jats:sub>) and phosphatidylinositol (PI)-specific phospholipase C (PLC) in the degranulation of human eosinophils and leukotriene (LT) C<jats:sub>4<\/jats:sub>synthesis. Activation with formyl-Met-Leu-Phe + cytochalasin B (fMLP\/B) caused a time-dependent release of eosinophil peroxidase (EPO) and LTC<jats:sub>4<\/jats:sub>, which was inhibited by pertussis toxin. By immunoblotting, eosinophil PLC-\u03b22 and -\u03b32 isoforms were identified, and PLC activation was measured as a function of inositol 1,4,5-trisphosphate concentration. Stimulated release of EPO and intracellular Ca<jats:sup>2+<\/jats:sup>concentration was inhibited by ET-18-OCH<jats:sub>3<\/jats:sub>, a PI-PLC inhibitor, whereas trifluoromethylketone (TFMK), a cPLA<jats:sub>2<\/jats:sub>blocker, had no inhibitory effect. Both TFMK and ET-18-OCH<jats:sub>3<\/jats:sub>attenuated stimulated arachidonate release and LTC<jats:sub>4<\/jats:sub>secretion, suggesting that activation of both PLC and cPLA<jats:sub>2<\/jats:sub>is essential for LTC<jats:sub>4<\/jats:sub>synthesis caused by fMLP\/B. The structurally unrelated protein kinase C inhibitors bisindolylmaleimide, Ro-31\u20138220, and Go-6976 all blocked fMLP\/B-induced EPO release but not LTC<jats:sub>4<\/jats:sub>secretion. 1,2-bis(2-Aminophenoxy)ethane- N,N,N\u2032,N\u2032- tetraacetic acid acetoxymethyl ester, an intracellular Ca<jats:sup>2+<\/jats:sup>chelator, suppressed both EPO release and LTC<jats:sub>4<\/jats:sub>secretion. We found that fMLP\/B-induced LTC<jats:sub>4<\/jats:sub>secretion from human eosinophils is regulated by PI-PLC through calcium-mediated activation of cPLA<jats:sub>2<\/jats:sub>. However, cPLA<jats:sub>2<\/jats:sub>does not regulate eosinophil degranulation.<\/jats:p>","DOI":"10.1152\/ajplung.2001.281.4.l844","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T20:51:42Z","timestamp":1514062302000},"page":"L844-L851","source":"Crossref","is-referenced-by-count":13,"title":["Regulation of eosinophil function by phosphatidylinositol-specific PLC and cytosolic PLA<sub>2<\/sub>"],"prefix":"10.1152","volume":"281","author":[{"given":"Akiko","family":"Sano","sequence":"first","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangdong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Sano","sequence":"additional","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nilda M.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan","family":"Boetticher","sequence":"additional","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan R.","family":"Leff","sequence":"additional","affiliation":[{"name":"Section of Pulmonary and Critical Care Medicine, Department of Medicine, and"},{"name":"Departments of Neurobiology, Pharmacology, Physiology, and Critical Care and Committees on Clinical Pharmacology and Cell Physiology, Division of the Biological Sciences, The University of Chicago, Chicago, Illinois 60637"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S0031-6997(25)06865-6","volume":"38","author":"Abdel-Latif AA.","year":"1986","journal-title":"Pharmacol Rev"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/140.1.125"},{"key":"B3","doi-asserted-by":"crossref","first-page":"5409","DOI":"10.1016\/S0021-9258(19)39374-3","volume":"265","author":"Channon JY","year":"1990","journal-title":"J Biol Chem"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90556-E"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/0929-7855(95)00012-F"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(92)90036-A"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1042\/cs0940461"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(89)90391-6"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.7.4007"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(91)90315-7"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(92)90022-Y"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(97)00613-8"},{"key":"B13","doi-asserted-by":"crossref","first-page":"3466","DOI":"10.4049\/jimmunol.147.10.3466","volume":"147","author":"Kita H","year":"1991","journal-title":"J Immunol"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.24.14816"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(91)90308-B"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)81214-1"},{"key":"B17","first-page":"559","volume":"64","author":"Kroegel C","year":"1988","journal-title":"Immunology"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.13.6147"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90666-E"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(98)70142-3"},{"key":"B21","first-page":"1320","volume":"57","author":"Mollinedo F","year":"1997","journal-title":"Cancer Res"},{"key":"B22","first-page":"303","volume":"61","author":"Moss J","year":"1988","journal-title":"Adv Enzymol Relat Areas Mol Biol"},{"key":"B23","doi-asserted-by":"crossref","first-page":"532","DOI":"10.4049\/jimmunol.138.2.532","volume":"138","author":"Owen WF","year":"1987","journal-title":"J Immunol"},{"key":"B24","first-page":"2835","volume":"52","author":"Powis G","year":"1992","journal-title":"Cancer Res"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1042\/bj2930255"},{"key":"B26","doi-asserted-by":"crossref","first-page":"25999","DOI":"10.1016\/S0021-9258(18)47150-5","volume":"269","author":"Roshak A","year":"1994","journal-title":"J Biol Chem"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1995.tb20737.x"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116436"}],"container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.2001.281.4.L844","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T04:44:44Z","timestamp":1751172284000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.2001.281.4.L844"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10,1]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,10,1]]}},"alternative-id":["10.1152\/ajplung.2001.281.4.L844"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.2001.281.4.l844","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10,1]]}}}