{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T16:46:51Z","timestamp":1709570811215},"reference-count":56,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2002,1,1]]},"abstract":"<jats:p>CD4<jats:sup>+<\/jats:sup>T cells have a critical role in the development of allergic pulmonary inflammation, including the recruitment of eosinophils to the airway lumen and interstitium. The expression of interleukin (IL)-5 by CD4<jats:sup>+<\/jats:sup>cells has, in particular, often been lionized as the central link between allergic inflammation and the concomitant expansion or recruitment of eosinophils. The mechanism(s) by which CD4<jats:sup>+<\/jats:sup>T cells mediates eosinophil recruitment was assessed with gene knockout mice deficient for T cells or T cell subtypes and a unique IL-5 transgenic mouse (line NJ.1726) that constitutively overexpresses this cytokine in the lung epithelium. Pulmonary IL-5 expression is significantly attenuated in T cell- and CD4<jats:sup>+<\/jats:sup>but not CD8<jats:sup>+<\/jats:sup>cell-deficient animals, suggesting an obvious explanation for the lack of eosinophils in the lungs of T cell-deficient and CD4(\u2212\/\u2212) mice. However, although the constitutive expression of IL-5 in the lung epithelium of NJ.1726 mice elicited an eosinophilia in the airway lumen of both naive and ovalbumin-treated mice, in the absence of CD4<jats:sup>+<\/jats:sup>cells, allergen-mediated eosinophil recruitment to the bronchoalveolar lavage fluid was abolished. Moreover, intranasal instillation of the potent eosinophil-specific chemokine eotaxin-2 was incapable of eliciting eosinophil recruitment in naive and ovalbumin-treated NJ.1726 CD4(\u2212\/\u2212) mice, suggesting that eosinophil trafficking during allergic inflammatory responses is a consequence of a CD4<jats:sup>+<\/jats:sup>cell-mediated event(s) in addition to IL-5 expression and the establishment of a pulmonary chemokine gradient.<\/jats:p>","DOI":"10.1152\/ajplung.2002.282.1.l99","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T21:49:17Z","timestamp":1514065757000},"page":"L99-L108","source":"Crossref","is-referenced-by-count":15,"title":["Ectopic expression of IL-5 identifies an additional CD4<sup>+<\/sup>T cell mechanism of airway eosinophil recruitment"],"prefix":"10.1152","volume":"282","author":[{"given":"Jeffrey R.","family":"Crosby","sequence":"first","affiliation":[{"name":"Divisions of Hematology\/Oncology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. H.","family":"Shen","sequence":"additional","affiliation":[{"name":"Divisions of Hematology\/Oncology and"},{"name":"Department of Respiratory Medicine, Second Hospital, Zhejiang University College of Medicine, Hangzhou 310009, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. T.","family":"Borchers","sequence":"additional","affiliation":[{"name":"Pulmonary Medicine, Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. P.","family":"Justice","sequence":"additional","affiliation":[{"name":"Divisions of Hematology\/Oncology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Ansay","sequence":"additional","affiliation":[{"name":"Divisions of Hematology\/Oncology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. J.","family":"Lee","sequence":"additional","affiliation":[{"name":"Pulmonary Medicine, Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. A.","family":"Lee","sequence":"additional","affiliation":[{"name":"Divisions of Hematology\/Oncology and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","first-page":"137","volume":"57","author":"Abu-Ghazaleh RI","year":"1992","journal-title":"Immunol Ser"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70256-8"},{"key":"B3","doi-asserted-by":"crossref","first-page":"711","DOI":"10.4049\/jimmunol.156.2.711","volume":"156","author":"Bates ME","year":"1996","journal-title":"J Immunol"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2001.281.3.L653"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199010113231505"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.4.2760"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.10.1737"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.9.1309"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12472082"},{"key":"B10","first-page":"271","volume":"69","author":"De Andres B","year":"1990","journal-title":"Immunology"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.10.5509"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.6.3385"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.1997.0308"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.1.195"},{"key":"B15","first-page":"35","volume":"42","author":"Frigas E","year":"1980","journal-title":"Lab Invest"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90462-8"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1995.1055"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.10.6.8003337"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1159\/000236978"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119045"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.19.9079"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.4.1719"},{"key":"B24","first-page":"679","volume":"51","author":"Henderson WR","year":"1984","journal-title":"Immunology"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1922.00110120002001"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117650"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)62267-3"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/145.2_Pt_2.S22"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.5.885"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.13.3.7654390"},{"key":"B31","doi-asserted-by":"crossref","first-page":"3601","DOI":"10.1182\/blood.V96.10.3601","volume":"96","author":"Larbi KY","year":"2000","journal-title":"Blood"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.12.2143"},{"key":"B33","doi-asserted-by":"crossref","first-page":"5526","DOI":"10.1016\/S0021-9258(18)91044-6","volume":"259","author":"Lee T","year":"1984","journal-title":"J Biol Chem"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1159\/000237023"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1007\/BF01977348"},{"key":"B36","doi-asserted-by":"crossref","first-page":"2664","DOI":"10.4049\/jimmunol.153.6.2664","volume":"153","author":"Minnicozzi M","year":"1994","journal-title":"J Immunol"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1038\/360225a0"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.4.2142"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1007\/BF02532741"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1990.70.2.427"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020531322556"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8945"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.57.030195.004143"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1172\/JCI4160"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1988.tb00743.x"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.3.449"},{"key":"B47","doi-asserted-by":"crossref","first-page":"2516","DOI":"10.4049\/jimmunol.161.5.2516","volume":"161","author":"Teixeira MM","year":"1998","journal-title":"J Immunol"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.7.4040"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.3.2005010"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)00056-X"},{"key":"B51","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.4049\/jimmunol.161.4.1962","volume":"161","author":"Walker C","year":"1998","journal-title":"J Immunol"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.24.7626"},{"key":"B53","doi-asserted-by":"crossref","first-page":"5592","DOI":"10.4049\/jimmunol.162.9.5592","volume":"162","author":"Wise JT","year":"1999","journal-title":"J Immunol"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1159\/000024038"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.1.43"},{"key":"B56","doi-asserted-by":"crossref","first-page":"5050","DOI":"10.4049\/jimmunol.158.10.5050","volume":"158","author":"Ying S","year":"1997","journal-title":"J Immunol"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.10.5839"}],"container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.2002.282.1.L99","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T05:48:31Z","timestamp":1693374511000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.2002.282.1.L99"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1,1]]},"references-count":56,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,1,1]]}},"alternative-id":["10.1152\/ajplung.2002.282.1.L99"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.2002.282.1.l99","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1,1]]}}}