{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:48:47Z","timestamp":1790063327954,"version":"4.0.1"},"reference-count":65,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:p> Although Cl<jats:sup>\u2212<\/jats:sup> transport in fetal lung is important for fluid secretion and normal lung development, the role of Cl<jats:sup>\u2212<\/jats:sup> transport in adult lung is not well understood. In physiological studies, the cystic fibrosis transmembrane regulator (CFTR) plays a role in fluid absorption in the distal air spaces of adult lung, and alveolar type II cells cultured for 5 days have the capacity to transport Cl<jats:sup>\u2212<\/jats:sup>. Although both alveolar type I and type II cells express CFTR, it has previously not been known whether type I cells transport Cl<jats:sup>\u2212<\/jats:sup>. We studied Cl<jats:sup>\u2212<\/jats:sup> uptake in isolated type I cells directly, using either radioisotopic tracers or halide-sensitive fluorescent indicators. By both methods, type I cells take up Cl<jats:sup>\u2212<\/jats:sup>. In the presence of \u03b2-adrenergic agonist stimulation, Cl<jats:sup>\u2212<\/jats:sup> uptake can be inhibited by CFTR antagonists. Type I cells express both the Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> anion exchanger AE2 and the voltage-gated Cl<jats:sup>\u2212<\/jats:sup> channels CLC5 and CLC2. Inhibitors of AE2 also block Cl<jats:sup>\u2212<\/jats:sup> uptake in type I cells. Together, these results demonstrate that type I cells are capable of Cl<jats:sup>\u2212<\/jats:sup> uptake and suggest that the effects seen in whole lung studies establishing the importance of Cl<jats:sup>\u2212<\/jats:sup> movement in alveolar fluid clearance may be, in part, the result of Cl<jats:sup>\u2212<\/jats:sup> transport across type I cells. <\/jats:p>","DOI":"10.1152\/ajplung.90466.2008","type":"journal-article","created":{"date-parts":[[2009,8,15]],"date-time":"2009-08-15T01:46:01Z","timestamp":1250300761000},"page":"L816-L827","source":"Crossref","is-referenced-by-count":19,"title":["Characteristics of Cl<sup>\u2212<\/sup> uptake in rat alveolar type I cells"],"prefix":"10.1152","volume":"297","author":[{"given":"Meshell","family":"Johnson","sequence":"first","affiliation":[{"name":"Department of Medicine,"},{"name":"San Francisco Veterans Affairs Medical Center, San Francisco, 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