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We now report on \u03b1<jats:sub>2<\/jats:sub>-, \u03b1<jats:sub>3<\/jats:sub>-, and \u03b2<jats:sub>1<\/jats:sub>-integrin and E-cadherin expression in normal human lung and in BPS and CCAM tissue previously shown to have abnormal HoxB5 expression and on the relationship of cell adhesion molecule expression to Hoxb5 regulation. \u03b1<jats:sub>2<\/jats:sub>-, \u03b1<jats:sub>3<\/jats:sub>-, and \u03b2<jats:sub>1<\/jats:sub>-integrins and E-cadherin expression in normal human lung and BPS and CCAM were evaluated using Western blot and immunohistochemistry. Fetal mouse lung fibroblasts with Hoxb5-specific siRNA downregulation were evaluated for \u03b1<jats:sub>2<\/jats:sub>-integrin protein levels by Western blot. Compared with normal human lung, a previously undetected \u03b1<jats:sub>2<\/jats:sub>-integrin isoform potentially lacking essential cytoplasmic sequences was significantly increased in BPS and CCAM, and \u03b1<jats:sub>2<\/jats:sub>-integrin spatial and cellular expression was more intense. E-cadherin protein levels were also significantly increased, whereas \u03b1<jats:sub>3<\/jats:sub>increased in CCAM compared with canalicular, but not with alveolar, stage lung. \u03b2<jats:sub>1<\/jats:sub>-integrin levels were unchanged. We conclude that in BPS and CCAM, altered \u03b1<jats:sub>2<\/jats:sub>-integrin cytoplasmic signaling contributes to abnormal cellular behavior in these lung lesions. Aberrant cell adhesion molecule and Hox protein regulation are likely part of the mechanism involved in the development of BPS and CCAM.<\/jats:p>","DOI":"10.1152\/ajplung.90618.2008","type":"journal-article","created":{"date-parts":[[2009,5,2]],"date-time":"2009-05-02T00:24:18Z","timestamp":1241223858000},"page":"L143-L152","source":"Crossref","is-referenced-by-count":25,"title":["Aberrant cell adhesion molecule expression in human bronchopulmonary sequestration and congenital cystic adenomatoid malformation"],"prefix":"10.1152","volume":"297","author":[{"given":"MaryAnn V.","family":"Volpe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunice","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason P.","family":"Ulm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian F.","family":"Gilchrist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Ralston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen T.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heber C.","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1002\/uog.1110"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1002\/uog.1112"},{"key":"R3","unstructured":"Aulicino MR, Reis ED, Dolgin SE, Unger PD, Shah KD.Intra-abdominal pulmonary sequestration exhibiting congenital cystic adenomatoid malformation: report of a case and review of literature.Arch Pathol Lab Med118: 1034\u20131037, 1994."},{"key":"R4","unstructured":"Barr LF, Cambell SE, Bochner BS, Dang CV.Association of the decreased expression of alpha3 beta 1 integrin with the altered cell: environmental interactions and enhanced soft agar cloning ability of c-myc overexpressing small cell cancer cells.Cancer Res58: 5537\u20135545, 1998."},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.04.011"},{"key":"R6","unstructured":"Bondos S.Variations on a theme: Hox and Wnt combinatorial regulation during animal development.Sci STKE38: 1\u20134, 2006."},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.04.010"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1042\/bst0320438"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(97)90383-3"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(98)90528-0"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64185-5"},{"key":"R12","doi-asserted-by":"crossref","unstructured":"Chen YP, O'Toole E, Shipley T, Forsyth J, LaFlamme SE, Yamada KM, Shattil SJ, Ginsberg MH.Inside-out signal transduction inhibited by isolated integrin cytoplasmic domains.J Biol Chem269: 18307\u201318310, 1994.","DOI":"10.1016\/S0021-9258(17)32306-2"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960529)66:5<692::AID-IJC18>3.0.CO;2-6"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1126\/science.7716514"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1007\/s100249900149"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1177\/002215549804600703"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.279.2.L224"},{"key":"R18","doi-asserted-by":"crossref","unstructured":"Daftary GS, Troy PJ, Bagot CN, Young SL, Taylor HS.Direct regulation of beta3-integrin subunit gene expression by HoxA10 in endometrial cells.Mol Endocrinol16: 571\u2013579, 2002.","DOI":"10.1210\/me.16.3.571"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(02)00368-X"},{"key":"R20","doi-asserted-by":"crossref","unstructured":"DeArcangelis A, Mark M, Kreidberg J, Sorokin L, Georges-Labouesse E.Synergestic activities of (alpha)3 and (alpha)6 integrins are required during apical ectodermal ridge formation and organogenesis in the mouse.Development126: 3957\u20133968, 1999.","DOI":"10.1242\/dev.126.17.3957"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1017\/S104795110600031X"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64042-4"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2007.124404"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1486806"},{"key":"R25","doi-asserted-by":"crossref","unstructured":"Hirai Y, Nose A, Kobayashi S, Takeichi M.Expression and role of E- and P-cadherin adhesion molecules in embryonic histogenesis. 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