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Here, we describe how environmentally relevant temperature changes affected mRNA levels of prl\n                    <jats:sub>188<\/jats:sub>\n                    and prl\n                    <jats:sub>177<\/jats:sub>\n                    and the release of immunoreactive prolactins from RPDs and dispersed PRL cells. When applied under isosmotic conditions (330 mosmol\/kgH\n                    <jats:sub>2<\/jats:sub>\n                    O), a 6\u00b0C rise in temperature stimulated the release of PRL\n                    <jats:sub>188<\/jats:sub>\n                    and PRL\n                    <jats:sub>177<\/jats:sub>\n                    from both RPDs and dispersed PRL cells under perifusion. When exposed to this same change in temperature, \u223c50% of dispersed PRL cells gradually increased in volume by \u223c8%, a response partially inhibited by the water channel blocker, mercuric chloride. Following their response to increased temperature, PRL cells remained responsive to a hyposmotic stimulus (280 mosmol\/kgH\n                    <jats:sub>2<\/jats:sub>\n                    O). The mRNA expression of transient potential vanilloid 4, a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -channel involved in hyposmotically induced PRL release, was elevated in response to a rise in temperature in dispersed PRL cells and RPDs at 6 and 24 h, respectively; prl\n                    <jats:sub>188<\/jats:sub>\n                    and prl\n                    <jats:sub>177<\/jats:sub>\n                    mRNAs were unaffected. Our findings indicate that thermosensitive PRL release is mediated, at least partially, through a cell-volume-dependent pathway similar to how osmoreceptive PRL release is achieved.\n                  <\/jats:p>","DOI":"10.1152\/ajpregu.00027.2022","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T08:00:39Z","timestamp":1650355239000},"page":"R609-R619","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Tilapia prolactin cells are thermosensitive osmoreceptors"],"prefix":"10.1152","volume":"322","author":[{"given":"Daniel W.","family":"Woo","sequence":"first","affiliation":[{"name":"Department of Human Nutrition, Food and Animal Sciences, University of Hawai\u2019i at M\u0101noa, Honolulu, Hawaii"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. H. 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