{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:59:07Z","timestamp":1740139147852,"version":"3.37.3"},"reference-count":69,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/501100000038","name":"Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2022,10,1]]},"abstract":"<jats:p> The sensing of environmental fluctuations and initiation of appropriate physiological responses is crucial to homeostasis. Neuroepithelial cells (NECs) in fishes are putative chemoreceptors, resembling mammalian Type I (glomus) cells, that respond in vitro to changes in O<jats:sub>2<\/jats:sub>, CO<jats:sub>2<\/jats:sub>, NH<jats:sub>3<\/jats:sub>, and pH. Cytosolic carbonic anhydrase (Ca17a) is thought to be involved in CO<jats:sub>2<\/jats:sub> sensing owing to its presence in NECs. Zebrafish ( Danio rerio) lacking functional Ca17a were generated via CRISPR\/Cas9 technology and used to assess the role of Ca17a in initiating the cardiorespiratory responses to elevated CO<jats:sub>2<\/jats:sub> (hypercapnia). Unfortunately, the homozygous knockout mutants ( ca17a<jats:sup>\u2212\/\u2212<\/jats:sup>) did not survive more than \u223c12\u201314 days postfertilization (dpf), restricting experiments to early developmental stages (4\u20138 dpf). Changes in ventilation ( f<jats:sub>V<\/jats:sub>) and cardiac ( f<jats:sub>H<\/jats:sub>) frequency in response to hypercapnia (1% CO<jats:sub>2<\/jats:sub>) in wild-type ( ca17a<jats:sup>+\/+<\/jats:sup>), heterozygous ( ca17a<jats:sup>+\/\u2212<\/jats:sup>) and ca17a<jats:sup>\u2212\/\u2212<\/jats:sup> fish were used to investigate Ca17a-dependent CO<jats:sub>2<\/jats:sub> sensing and downstream signaling. Wild-type fish exhibited hyperventilation during hypercapnia as indicated by an increase in f<jats:sub>V<\/jats:sub>. In the ca17a<jats:sup>\u2212\/\u2212<\/jats:sup> fish, the hyperventilatory response was attenuated markedly but only at 8 dpf. Hypercapnic tachycardia was observed for all genotypes and did not appear to be influenced by the absence of Ca17a. Interestingly, ca17a<jats:sup>\u2212\/\u2212<\/jats:sup> fish exhibited a significantly lower resting f<jats:sub>H<\/jats:sub> that became more pronounced as the fish aged. The decrease in resting f<jats:sub>H<\/jats:sub> was prevented (\u201crescued\u201d) when ca17a<jats:sup>\u2212\/\u2212<\/jats:sup> embryos were injected with ca17a mRNA. Collectively, the results of this study support a role for Ca17a in promoting hyperventilation during hypercapnia in larval zebrafish and suggest a previously unrecognized role for Ca17a in determining resting heart rate. <\/jats:p>","DOI":"10.1152\/ajpregu.00050.2022","type":"journal-article","created":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T11:56:49Z","timestamp":1661169409000},"page":"R532-R546","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Role of cytosolic carbonic anhydrase Ca17a in cardiorespiratory responses to CO<sub>2<\/sub> in developing zebrafish (<i>Danio rerio)<\/i>"],"prefix":"10.1152","volume":"323","author":[{"given":"E.","family":"Kunert","sequence":"first","affiliation":[{"name":"Department of Biology, University of Ottawa, Ottawa, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3782-1641","authenticated-orcid":false,"given":"W.","family":"Joyce","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ottawa, Ottawa, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1455-1439","authenticated-orcid":false,"given":"Y. 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