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RT-PCR and immunohistochemistry were performed to study ENaC and MR expression at the mRNA and protein levels, respectively. Both mRNA and protein for \u03b1-, \u03b2-, and \u03b3-ENaC subunits and MR were found to be expressed in the rat brain. All three ENaC subunits and MR were present in the supraoptic nucleus, magnocellular paraventricular nucleus, hippocampus, choroid plexus, ependyma, and brain blood vessels, suggesting the presence of multimeric channels and possible regulation by mineralocorticoids. In most cortical areas, thalamus, amygdala, and suprachiasmatic nucleus, notable expression of \u03b3-ENaC was undetectable, whereas \u03b1- and \u03b2-ENaC were abundantly expressed pointing to the possibility of a heterogeneous population of channels. The findings suggest that stoichiometrically different populations of ENaC may be present in both epithelial and neural components in the brain, which may contribute to regulation of cerebrospinal fluid and interstitial Na<jats:sup>+<\/jats:sup> concentration as well as neuronal excitation. <\/jats:p>","DOI":"10.1152\/ajpregu.00063.2005","type":"journal-article","created":{"date-parts":[[2005,9,3]],"date-time":"2005-09-03T00:34:00Z","timestamp":1125707640000},"page":"R1787-R1797","source":"Crossref","is-referenced-by-count":123,"title":["Distribution of epithelial sodium channels and mineralocorticoid receptors in cardiovascular regulatory centers in rat brain"],"prefix":"10.1152","volume":"289","author":[{"given":"Md Shahrier","family":"Amin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erona","family":"Reza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stewart C.","family":"Whitman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balwant S.","family":"Tuana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frans H. 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