{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T04:10:45Z","timestamp":1770264645394,"version":"3.49.0"},"reference-count":75,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p>Norepinephrine (NE) microdialyzed in the preoptic area (POA) raises core temperature (T<jats:sub>c<\/jats:sub>) via 1) \u03b1<jats:sub>1<\/jats:sub>-adrenoceptors (AR), quickly and independently of POA PGE<jats:sub>2<\/jats:sub>, and 2) \u03b1<jats:sub>2<\/jats:sub>-AR, after a delay and PGE<jats:sub>2<\/jats:sub>dependently. Since systemic lipopolysaccharide (LPS) activates the central noradrenergic system, we investigated whether preoptic NE mediates LPS fever. We injected LPS (2 \u03bcg\/kg iv) in guinea pigs prepared with intra-POA microdialysis probes and determined POA cerebrospinal (CSF) NE levels. We similarly microdialyzed prazosin (\u03b1<jats:sub>1<\/jats:sub>blocker, 1 \u03bcg\/\u03bcl), yohimbine (\u03b1<jats:sub>2<\/jats:sub>blocker, 1 \u03bcg\/\u03bcl), SC-560 [cyclooxygenase (COX)-1 blocker, 5 \u03bcg\/\u03bcl], acetaminophen (presumptive COX-1v blocker, 5 \u03bcg\/\u03bcl), or MK-0663 (COX-2 blocker, 0.5 \u03bcg\/\u03bcl) in other animals before intravenous LPS and measured CSF PGE<jats:sub>2<\/jats:sub>. All of the agents were perfused at 2 \u03bcg\/min for 6 h. T<jats:sub>c<\/jats:sub>was monitored constantly. POA NE peaked within 30 min after LPS and then returned to baseline over the next 90 min. T<jats:sub>c<\/jats:sub>increased within 12 min to a first peak at \u223c60 min and to a second at \u223c150 min and then declined over the following 2.5 h. POA PGE<jats:sub>2<\/jats:sub>followed a concurrent course. Prazosin pretreatment eliminated the first T<jats:sub>c<\/jats:sub>rise but not the second; PGE<jats:sub>2<\/jats:sub>rose normally. Yohimbine pretreatment did not affect the first T<jats:sub>c<\/jats:sub>rise, which continued unchanged for 6 h; the second rise, however, was absent, and PGE<jats:sub>2<\/jats:sub>levels did not increase. SC-560 and acetaminophen did not alter the LPS-induced PGE<jats:sub>2<\/jats:sub>and T<jats:sub>c<\/jats:sub>rises; MK-0663 prevented both the late PGE<jats:sub>2<\/jats:sub>and T<jats:sub>c<\/jats:sub>rises. 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